[1]刘浩,曹友德,叶维霞,等.miR-206抑制乳腺癌MDA-MB-231细胞Cdc42蛋白表达及其对细胞骨架的影响[J].第三军医大学学报,2010,32(06):568-571.
 Liu Hao,Cao Youde,YE Weixia,et al.miR-206 inhibits Cdc42 protein expression, invasion and migration of MDA-MB-231 cells[J].J Third Mil Med Univ,2010,32(06):568-571.
点击复制

miR-206抑制乳腺癌MDA-MB-231细胞Cdc42蛋白表达及其对细胞骨架的影响(/HTML )
分享到:

《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
32卷
期数:
2010年第06期
页码:
568-571
栏目:
论著
出版日期:
2010-03-30

文章信息/Info

Title:
miR-206 inhibits Cdc42 protein expression, invasion and migration of MDA-MB-231 cells
作者:
刘浩曹友德叶维霞孙阳阳
重庆医科大学基础医学院病理学教研室,分子医学与肿瘤研究中心
Author(s):
Liu Hao Cao Youde YE Weixia Sun Yangyang
Department of Pathology, Molecular Medicine and Cancer Research Center, Chongqing Medical University, Chongqing, 400016, China
关键词:
乳腺肿瘤侵袭转移miR-206Cdc42
Keywords:
breast tumor invasion migration miR-206 Cdc42
分类号:
R342.22;R73-36;R737.9
文献标志码:
A
摘要:
目的   探讨miR-206对MDA-MB-231乳腺癌细胞Cdc42表达的调控及对细胞骨架的影响。   方法   采用LipofectamineTM 2000转染miR-206进入MDA-MB-231细胞,48 h后Western blot检测转染后乳腺癌细胞Cdc42、MMP-2和MMP-9蛋白质表达。用免疫荧光显微镜观察细胞丝状伪足的改变,进一步用侵袭迁移实验检测转染前后细胞侵袭力和迁移力的变化。   结果   Western blot检测Cdc42、MMP-2和MMP-9在转染前后的表达结果,其条带灰度值与阴性对照组相比明显下降(P<0.05);细胞免疫荧光计数每个细胞平均丝状伪足数,空白对照组为(14.99±5.53),表皮生长因子(EGF)组为(23.59±3.92),miR-206转染组为(9.45±3.59),miR-206+EGF组为(11.77±2.85)。与空白对照组和EGF组比较,miR-206转染组或miR-206+EGF组细胞丝状伪足明显减少(P<0.05)。侵袭实验结果显示,小室膜下的细胞数量空白对照组为(311.7±23.5),miR-206转染组为(65.0±13.9),二者差异有统计学意义(P<0.01)。迁移实验结果显示,小室膜下的细胞数量空白对照组为(793.0±76.3),而miR-206转染组为(415.3±20.0),二者差异有统计学意义(P<0.01)。   结论   miR-206能抑制MDA-MB-231细胞Cdc42的表达和细胞丝状伪足的形成,并且能抑制细胞的侵袭迁移能力。
Abstract:
Objective   To investigate whether miR-206 can regulate Cdc42 protein expression and cytoskeleton remodeling.    Methods   miR-206 was transfected into MDA-MB-231 cells using LipofectamineTM 2000, and the transfected cells were harvested in 48 h. Western blot analysis was used to detect the protein expressions of Cdc42, MMP-2 and MMP-9. Actin-Tracker Green was used to stain F-actin and filopodia was observed by fluorescent microscopy. Invasion assay and Transwell migration assay were taken to examine the invasive and migratory ability of cells before and after the transfection respectively.    Results   Protein expressions of Cdc42, MMP-2 and MMP-9 were all down-regulated (P<0.01). The number of filopodia per cell in the blank group (untransfected group) was 14.99±5.53, in EGF group was 23.59±3.92, miR-206 group was (9.45±3.59), and miR-206+EGF group was 11.77±2.85. The filopodia numbers in miR-206 and miR206+EGF groups was much less than those in the blank group and EGF group (P<0.05). The invasion and migration of miR-206 transfected MDA-MB-231 cells (65.0±13.9) were significantly lower than MDA-MB-231 cells [blank control, 311.7±23.5, P<0.05]; Transwell migratory assay indicated that there were more migrated cells in blank control group than in miR-206 group [793.0±76.3 vs 415.3±20.0, P<0.05].    Conclusion   miR-206 downregulates Cdc42 protein expression in MDA-MB-231 cells, inhibits the formation of filopodia, and thus inhibits the invasion and migration efficiency of the cells.

参考文献/References:

刘浩,曹友德,叶维霞,等. miR-206抑制乳腺癌MDA-MB-231细胞Cdc42蛋白表达及其对细胞骨架的影响[J]. 第三军医大学学报,2010,32(6):568-571.

相似文献/References:

[1]范婷婷,唐良萏.Nek2基因沉默对卵巢癌SKOV3细胞侵袭能力的影响[J].第三军医大学学报,2012,34(15):1514.
 Fan Tingting,Tang Liangdan.Silencing Nek2 via RNAi suppresses invasiveness in ovarian cancer SKOV3 cells[J].J Third Mil Med Univ,2012,34(06):1514.
[2]陈庆峰,牛兆河,刘翠翠,等.鼠-人嵌合抗粘蛋白1 IgG1全抗体的构建及其功能初步分析[J].第三军医大学学报,2012,34(17):1771.
 Chen Qingfeng,Niu Zhaohe,Liu Cuicui,et al.Construction and characterization of a mouse-human chimeric anti-mucin1 IgG antibody[J].J Third Mil Med Univ,2012,34(06):1771.
[3]庞林宾,王洪林,王晔飞,等.JAK2/STAT3通路在肝癌细胞侵袭及血管生成拟态中的作用[J].第三军医大学学报,2012,34(18):1862.
 Pang Linbin,Wang Honglin,Wang Yefei,et al.Role JAK2/STAT3 signaling pathway in invasion and vascular mimicry of liver cancer cells[J].J Third Mil Med Univ,2012,34(06):1862.
[4]黄士隋,史良会,黄广岩.RNA干扰下调LAT1表达对胃腺癌SGC-7901细胞增殖、侵袭、转移及细胞周期的影响[J].第三军医大学学报,2013,35(04):320.
 Huang Shisui,Shi Lianghui,Huang Guangyan.Effect of RNA interference targeting LAT1 on proliferation, migration and invasion of SGC7901 cells[J].J Third Mil Med Univ,2013,35(06):320.
[5]党微旗,唐浩,曹红,等.可调控STAT3干扰载体抑制BIU-87细胞侵袭的体外研究[J].第三军医大学学报,2013,35(05):400.
 Dang Weiqi,Tang Hao,Cao Hong,et al.Effect of CRE-dependent RNA interference targeting STAT3 on invasion and migration in human bladder cancer BIU-87 cells[J].J Third Mil Med Univ,2013,35(06):400.
[6]张彦,郑晓东,唐鹏,等.Sphk1基因对人乳腺癌MCF-7细胞增殖、凋亡和迁移能力的影响[J].第三军医大学学报,2012,34(21):2141.
 Zhang Yan,Zheng Xiaodong,Tang Peng,et al.Sphk1 interference suppresses proliferation, apoptosis and migration in human MCF-7 breast cancer cells[J].J Third Mil Med Univ,2012,34(06):2141.
[7]王杨,陈彬,姜晓梅,等.睾酮对乳腺癌细胞中FEN1表达的影响[J].第三军医大学学报,2013,35(02):95.
 Wang Yang,Chen Bin,Jiang Xiaomei,et al.Effect of testosterone on FEN1 expression in breast cancer cells[J].J Third Mil Med Univ,2013,35(06):95.
[8]李昌秀,曹友德.乳腺癌组织COX-2、VEGF-C的表达与淋巴结转移的关系[J].第三军医大学学报,2007,29(20):1964.
 LI Chang-xiu,CAO You-de.Correlation of COX-2 and VEGF-C expressions in breast carcinoma with lymph node metastasis[J].J Third Mil Med Univ,2007,29(06):1964.
[9]周艳,姜军,张毅,等.雌激素受体β及其剪切变异体表达与雌激素受体阻滞剂治疗耐药的关系[J].第三军医大学学报,2007,29(20):1999.
 ZHOU Yan,JIANG Jun,ZHANG Yi,et al.Relationship of estrogen receptor-beta and its isoform expressions with tamoxifen resistance in human breast cancer[J].J Third Mil Med Univ,2007,29(06):1999.
[10]娄四龙,霍钢,郑履平,等.PTTG、bFGF和P53的表达与垂体腺瘤侵袭性的关系[J].第三军医大学学报,2007,29(18):1790.
 LOU Si-long,HUO Gang,ZHENG Lu-ping,et al.Relationship of PTTG, bFGF, P53 expressions with invasiveness of pituitary adenoma[J].J Third Mil Med Univ,2007,29(06):1790.

更新日期/Last Update: 2010-03-24