[1]杨春蓉,张徽,黃伟,等.PDTC联合紫杉醇降低MDA-MB-231细胞增殖侵袭能力[J].第三军医大学学报,2010,32(06):576-579.
 Yang Chunrong,Zhang Hui,Huang Wei,et al.Combination of pyrrolidine dithiocarbamate and paclitaxel suppresses proliferation and invasion of MDA-MB-231 cells[J].J Third Mil Med Univ,2010,32(06):576-579.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
32卷
期数:
2010年第06期
页码:
576-579
栏目:
论著
出版日期:
2010-03-30

文章信息/Info

Title:
Combination of pyrrolidine dithiocarbamate and paclitaxel suppresses proliferation and invasion of MDA-MB-231 cells
作者:
杨春蓉张徽黃伟刘琼
重庆医科大学:分子医学与肿瘤研究中心,病理学教研室
Author(s):
Yang Chunrong Zhang Hui Huang Wei Liu Qiong
hongqing Research Centre of Molecular Medicine and Cancer, Department of Pathology, College of Basic Medical Sciences, Chongqing Medical University, Chongqing, 400016, China
关键词:
NF-κBPDTC乳腺癌MMP-9侵袭转移
Keywords:
NF-κB pyrrolidine dithiocarbamate breast cancer MMP-9 invasion and metastasis
分类号:
R730.23;R737.9;R979.1
文献标志码:
A
摘要:
目的   探讨核因子-κB(NF-κB)抑制剂——吡咯烷二硫代氨基甲酸盐(pyrrolidine dithiocarbamate,PDTC)联合紫杉醇(Paclitaxel)对人乳腺癌MDA-MB-231细胞增殖侵袭能力的影响。   方法   MTT及FCM法测定细胞增殖和周期变化,RT-PCR检测细胞NF-κB p65 mRNA的变化,Western blot检测细胞NF-κB p65、MMP-9及TIMP-1蛋白表达变化,侵袭、迁移和黏附实验测定细胞侵袭转移能力的改变。   结果   PDTC联合紫杉醇能明显抑制肿瘤细胞生长(P<0.05),细胞周期阻滞在G1/G0期,并可抵消紫杉醇对NF-κB的激活,使NF-κB p65 mRNA及蛋白的表达均降低(P<0.05)。PDTC降低MDA-MB-231细胞的侵袭转移能力,与紫杉醇联合应用后作用增强(P<0.01)。   结论   PDTC联合紫杉醇能降低乳腺癌MDA-MB-231细胞的侵袭转移能力,其机制可能与PDTC抑制NF-κB的表达相关。
Abstract:
Objective   To investigate the effect of the nuclear factor-κB (NF-κB) inhibitor, pyrrolidine dithiocarbamate (PDTC) and paclitaxel on the proliferation and invasion of human breast cancer cell line MDA-MB-231.    Methods   The MDA-MB-231 cells were treated with PDTC (100 μmol/L) or paclitaxel (1 μmol/L), or combined treatment. The effect of proliferation was analyzed by MTT assay. The cell cycle was evaluated by flow cytometry. RT-PCR was used to detect the expression of NF-κB p65 mRNA. Western blotting were used to determing the expression of NF-κB p65, MMP-9 and TIMP-1 in MDA-MB-231 cells after treatment. The invasion ability of MDA-MB-231 cells was tested ty the invasion, migration and cell adhesion assay.    Results   The cell growth was significantly slowed down and the cell cycle was arrested at G0/G1 phase after combined treatment. The expression of NF-κB p65 and MMP-9 was downregulated (P<0.05) compared with paclitaxel treatment group, and the invasion ability of MDA-MB-231 cells was decreased after combined treatment (P<0.01).    Conclusion   PDTC combined with paclitaxel effectively inhibits cell proliferation, induces cell cycle arrest, and decreases cell invasion ability of human breast cancer MDA-MB-231 cells. Its mechanism may be associated with PDTC inhibiting the NF-κB-related gene expression.

参考文献/References:

杨春蓉,张徽,黃伟,等. PDTC联合紫杉醇降低MDA-MB-231细胞增殖侵袭能力[J]. 第三军医大学学报,2010,32(6):576-579.

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更新日期/Last Update: 2010-03-24