[1]张雨平,黄其林,赵聪敏,等.不同发育阶段大鼠脑髓鞘化的研究[J].第三军医大学学报,2009,31(22):2189-2192.
 ZHANG Yu-ping,HUANG Qi-lin,ZHAO Cong-min,et al.Myelination of rat brain at different developmental stages[J].J Third Mil Med Univ,2009,31(22):2189-2192.
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不同发育阶段大鼠脑髓鞘化的研究(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
31卷
期数:
2009年第22期
页码:
2189-2192
栏目:
专题报道
出版日期:
2009-11-30

文章信息/Info

Title:
Myelination of rat brain at different developmental stages
作者:
张雨平黄其林赵聪敏汤金梁王亚丽
第三军医大学新桥医院:儿科,神经外科,全军癫痫病诊治中心,病理科
Author(s):
ZHANG Yu-ping HUANG Qi-lin ZHAO Cong-min TANG JIN-liang WANG Ya-li
Department of Paediatrics, Department of Neurosurgery, Department of Pathology, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China
关键词:
大鼠髓鞘化发育
Keywords:
rat brain myelination development
分类号:
R-332;R322.81;R339.3
文献标志码:
A
摘要:
目的  研究大鼠不同发育阶段脑髓鞘化的发展及规律。  方法  应用LFB髓鞘染色、MBP免疫组化及Wes-tern blot观察不同发育阶段大鼠脑髓鞘的分布及变化。  结果  LFB及MBP免疫组化染色E18、P0、P2均未见着色,P7大鼠胼胝体中部少量LFB着色,P15两种染色均呈阳性,P30着色加深,与P90、P720类似。通过Western blot检测MBP蛋白在孕中后期已有表达,出生后随日龄增长逐渐增加,至老年期表达量最高。  结论  大鼠脑髓鞘化从P15已经开始,P30接近成熟;观察髓鞘的最佳时间在30 d以后。
Abstract:
Objective   To investigate the myelination of rat brain at different developmental stages.    Methods   Luxol fast blue staining, immunohistochemistry and Western blotting were applied to determine the distribution and alternation of myelin sheath in the brains of SD rats at different developmental stages.     Results   Negative LFB and MBP staining were shown at the stages of embryonicage 18 d (E18), postnatal age 0 d (P0) and P2. At P7, the corpus callosum was weakly stained by LFB. The positive stain of LFB and MBP occurred in P15 on the corpus callosum. With the development going on, the stain turned out stronger, especially in P30, which was similar with that in P90 and P720. The results of Western blot analysis showed the expression of MBP in rat brain was gradually increased with the development of rat brain.     Conclusion   The myelination starts before P15 in rat brain and becomes mature in P30. The optimal observation time of myelin is 30 d born after.

参考文献/References:

张雨平,黄其林,赵聪敏,等.不同发育阶段大鼠脑髓鞘化的研究[J]. 第三军医大学学报,2009,31(22):2189-2192.

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更新日期/Last Update: 2009-11-23