[1]尹晓娟,周冬翠,安育林,等.高压氧在新生鼠缺氧缺血性脑损伤神经修复中的作用机制[J].第三军医大学学报,2009,31(22):2175-2177.
 YIN Xiao-juan,ZHOU Dong-cui,AN Yu-lin,et al.Function mechanism of HBO in neural repairation of neonatal rats after hypoxic-ischemic brain damage[J].J Third Mil Med Univ,2009,31(22):2175-2177.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
31卷
期数:
2009年第22期
页码:
2175-2177
栏目:
专题报道
出版日期:
2009-11-30

文章信息/Info

Title:
Function mechanism of HBO in neural repairation of neonatal rats after hypoxic-ischemic brain damage
作者:
尹晓娟周冬翠安育林罗分平封志纯
北京军区总医院附属八一儿童医院新生儿医学中心
Author(s):
YIN Xiao-juan ZHOU Dong-cui AN Yu-lin LUO Fen-ping FENG Zhi-chun
Centre of Neonatology,Bayi Children’s Hospital, General Hospital of Beijing Military Command, Beijing 100700, China
关键词:
新生鼠缺氧缺血性脑损伤高压氧骨形成蛋白4神经巢蛋白
Keywords:
neonatal rat hypoxic-ischemic brain damage hyperbaric oxygenbone morphogenetic protein-4nestin
分类号:
R459.6;R741.02;R743.31
文献标志码:
A
摘要:
目的  探讨高压氧(hyperbaric oxygen, HBO)在新生鼠缺氧缺血性脑损伤(hypoxic-ischemic brain damage, HIBD)神经修复中的作用机制。  方法  新生7日龄SD乳鼠30只,随机分为HIBD+HBO干预组、HIBD组和正常对照组,每组10只。干预组于建模后予以HBO治疗,连续7 d。采用免疫组化技术检测各组骨形态发生蛋白4(BMP-4)及神经巢蛋白(nestin)在海马的表达;采用原位杂交技术检测各组BMP-4 及nestin mRNA在海马的表达;采用TUNEL方法检测各组神经细胞凋亡。  结果  3组中,干预组BMP-4蛋白在海马的表达最多,HIBD组BMP-4蛋白在海马的表达最少;干预组nestin蛋白在海马的表达最多,HIBD组nestin蛋白在海马的表达最少;干预组BMP-4 mRNA在海马的表达最多,HIBD组BMP-4 mRNA在海马的表达最少;干预组nestin mRNA在海马的表达最多,HIBD组nestin mRNA在海马的表达最少;HIBD组凋亡神经细胞最多,HBO干预组凋亡神经细胞与HIBD组差异有统计学意义(P<0.01)。   结论  HBO对HIBD具有神经修复作用,其作用机制可能与促进BMP-4及nestin在海马的表达以及抑制神经细胞的凋亡有关。
Abstract:
Objective   To investigate the function mechanism of hyperbaric oxygen(HBO) in neural repairation of neonatal rats after hypoxic-ischemic brain damage (HIBD).    Methods   Thirty 7-day-old rats were randomly divided into control group, HIBD group and HIBD+HBO group, with 10 rats in each. Bone morphogenetic protein-4(BMP-4)and nestin protein in the hippocampus were determined with immunohistochemical method while their messenger ribonucleic acid (mRNA) were determined with in situ hybridization. Apoptosis of nerve cells was determined with TUNEL.    Results   Expression of BMP-4 and nestin protein in the hippocampus was the highest in HIBD+HBO group while their expression in HIBD group was lower than that in control group. Expression of BMP-4 and nestin mRNA in the hippocampus was the highest in HIBD+HBO group while their expression in HIBD group was lower than that in control group. Apoptosis of neural cells in HIBD group was the highest among the 3 groups while apoptosis of neural cells in HIBD+HBO group was lower than that in HIBD group(P<0.01).    Conclusion   HBO plays a role in nerve reparation, probably by promoting expression of BMP-4 and nestin in the hippocampus and inhibiting apoptosis of neural cells.

参考文献/References:

尹晓娟,周冬翠,安育林,等.高压氧在新生鼠缺氧缺血性脑损伤神经修复中的作用机制[J]. 第三军医大学学报,2009,31(22):2175-2177.

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更新日期/Last Update: 2009-11-23