[1]姚凤华,何娅妮,詹俊,等.内质网应激在急性缺血性大鼠肾损伤中的作用[J].陆军军医大学学报(原第三军医大学学报),2009,31(11):1002-1004.
 YAO Feng-hua,HE Ya-ni,ZHAN Jun,et al.Role of endoplasmic reticulum stress in acute ischemic renal injury in rats[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(11):1002-1004.
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陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
31卷
期数:
2009年第11期
页码:
1002-1004
栏目:
论著
出版日期:
2009-06-15

文章信息/Info

Title:
Role of endoplasmic reticulum stress in acute ischemic renal injury in rats
作者:
姚凤华何娅妮詹俊冀志勇
第三军医大学大坪医院野战外科研究所肾内科
Author(s):
YAO Feng-hua HE Ya-ni ZHAN Jun JI Zhi-yong
Department of Nephrology, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing 400042, China
关键词:
内质网应激GRP78肾缺血再灌注
Keywords:
endoplasmic reticulum stress GRP78 renal ischemia/reperfusion
分类号:
R363.2;R364.12;R692
文献标志码:
A
摘要:
目的   研究大鼠肾脏内质网应激(endoplasmic reticulum stress,ERS)与急性缺血性肾损伤(acute kidney injury,AKI)的关系。   方法   30只雄性SD大鼠采用随机数字表法分为假手术组(n=6)和模型组(n=24),模型组下设缺血40 min再灌注1、6、12、24 h 4个时相点,每个时相点6只。采用自动生化分析仪检测大鼠血肌酐、尿素氮水平。采用PAS染色检测肾小管损伤情况,免疫组化技术检测肾组织ERS标志物GRP78蛋白表达。   结果   ①血肌酐在缺血再灌注(ischemic/reperfusion,I/R)后1 h明显升高,12 h达高峰,24 h下降(P<0.05)。肾小管损伤评分在I/R后1 h升高,12 h达高峰,24 h下降(P<0.05)。②肾组织中GRP78蛋白表达在I/R后6 h升高达高峰,至12 h仍持续较高水平(P<0.05),24 h时下降。③GRP78表达与肾小管损伤呈显著正相关性(r=0.671,P<0.05),也与血肌酐水平呈显著正相关性(r=0.559,P<0.05)。   结论   肾I/R启动了ERS,并且ERS与肾小管损伤、血肌酐水平呈正相关关系。ERS可能在AKI中起重要作用。
Abstract:
Objective   To observe the relationship between endoplasmic reticulum stress and ischemia acute renal injury.    Methods   The renal ischemia and reperfusion model was developed by bilateral clamping of the renal pedicle for 40 min and reperfusing for 1, 6,12 and 24 h, respectively. Blood SCr and BUN were detected. Renal tubular injury was measured by PAS staining. The expression of ERS marker ,GRP78 protein, in the ischemic renal tissue was detected by immunohistochemistry.    Results   Scr increased initially at I/R 1 h, reaching a peak at I/R 12 h, and decayed at I/R 24 h . The score of renal tubular injury increased at I/R 1 h, reaching a peak at I/R 12 h, and decayed at I/R 24 h. The expression of GRP78 protein reached the peak at I/R 6 h, maintaining a high level at I/R 12 h, and declined at I/R 24 h. The expression of GRP78 protein had positive correlation with renal tubular injury, and Scr.    Conclusion   ERS is started after renal I/R in rats. It may play an important role in AKI.

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更新日期/Last Update: 2009-05-21