[1]胡晓红,段蕴铀,李毅,等.胸部开放伤后海水和淡水浸泡对肺损伤的作用比较[J].第三军医大学学报,2009,31(21):2090-2093.
 HU Xiao-hong,DUAN Yun-you,LI Yi,et al.Differences between acute lung injury induced by immersion in seawater and in freshwater in dogs after open chest trauma[J].J Third Mil Med Univ,2009,31(21):2090-2093.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
31卷
期数:
2009年第21期
页码:
2090-2093
栏目:
论著
出版日期:
2009-11-15

文章信息/Info

Title:
Differences between acute lung injury induced by immersion in seawater and in freshwater in dogs after open chest trauma
作者:
胡晓红段蕴铀李毅薛志强孟继光
军事医学科学院研究生队;海军总医院呼吸科;解放军总医院胸外科
Author(s):
HU Xiao-hong DUAN Yun-you LI Yi XUE Zhi-qiang MENG Ji-guang
Postgraduate College, Academy of Military Medical Sciences, Beijing 100850; Department of Respiratory Diseases, Naval General Hospital, Beijing 100048;Department of Thoracic Surgery, Chinese PLA General Hospital, Beijing 100853, China
关键词:
急性肺损伤淡水海水胸部开放伤浸泡
Keywords:
acute lung injury freshwater seawater open chest trauma immersion
分类号:
R563;R655;R835.3
文献标志码:
A
摘要:
目的   比较胸部开放伤后胸腔海水与淡水浸泡对肺损伤的作用 。   方法   24只实验犬致胸部开放伤后分成对照组、海水组和淡水组。海水组和淡水组伤后10 min内经伤口分别灌入海水和淡水;观察胸部开放伤后0、2、4、6、8 h的生命体征,测渗透压、血浆炎症因子变化。伤后8 h取肺组织观察病理学和炎症因子变化。   结果   肺泡通透指数: 海水组较淡水组明显升高(P<0.05),淡水组与对照组比较无统计学差异(P>0.05);IL-1β、IL-8: 海水组较淡水组升高(P<0.05),淡水组较对照组升高(P<0.05);血管性血友病因子(von Willnbrang factor, vWf):海水组较淡水组、对照组升高(P<0.05),淡水组较对照组无差异。相关分析显示:血浆渗透压与肺泡通透性指数、炎症因子IL-1β、IL-8正相关(P<0.05)。   结论   海水较淡水对开放伤后肺组织的损伤程度更严重,可能与海水高渗加重肺血管通透性和过度炎症反应有关。
Abstract:
Objective   To compare the differences between acute lung injury (ALI) induced by seawater-immersion and freshwater-immersion after open chest trauma.   Methods   Twenty-four dogs were randomly divided into 3 groups, ALI model group (control), seawater immersion group (group SW), and freshwater immersion group (group FW). ALI model was established by cutting an incision of 0.5 cm at the 4th intercostal space. Then artificial seawater or pure water (35 ml/kg) was perfused into the incision for the later 2 groups. The pathological changes were evaluated by light microscopy, hemotoxin and eosin (HE) staining in 8 h after perfusion. In 0, 2, 4, 6 and 8 h after trauma, the plasma samples were collected to test the levels of IL-1β, IL-8 and von Willnbrang factor (vWF) with ELISA. The amount of total protein in plasma and bronchoalveolar lavage fluid (BALF) were measured.   Results   Compared with group FW, the pulmonary penetration index, the concentrations of IL-1β, IL-8 and vWF in the plasma and lung tissue in group SW were markedly higher. They were significantly positively correlated with the plasma osmotic  pressure  (P>0.05).   Conclusion   Despite immersing in seawater and in freshwater worsen the ALI after open chest trauma, the ALI induced by seawater-immersion is severer than that caused by freshwater-immersion. It is attributed to higher plasma osmo-tic pressure and abundance of salts after seawater immersion, which increasing the pulmonary penetration index and aggravating the inflammatory response.

参考文献/References:

胡晓红, 段蕴铀, 李毅, 等. 胸部开放伤后海水和淡水浸泡对肺损伤的作用比较[J]. 第三军医大学学报,2009,31(21):2090-2093.

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更新日期/Last Update: 2009-11-02