[1]蓝建华,王德林,杨宗珂,等.膀胱移行细胞癌中整合素连接激酶的表达及意义[J].第三军医大学学报,2009,31(13):1243-1245.
 LAN Jian-hua,WANG De-lin,YANG Zong-ke,et al.Expression of integrin-linked kinase in human bladder transitional cell carcinoma and the related significance[J].J Third Mil Med Univ,2009,31(13):1243-1245.
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膀胱移行细胞癌中整合素连接激酶的表达及意义(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
31卷
期数:
2009年第13期
页码:
1243-1245
栏目:
专题报道
出版日期:
2009-07-15

文章信息/Info

Title:
Expression of integrin-linked kinase in human bladder transitional cell carcinoma and the related significance
作者:
蓝建华王德林 杨宗珂 李曾
重庆医科大学附属第一医院泌尿外科
Author(s):
LAN Jian-hua WANG De-lin YANG Zong-ke LI Zeng
Department of Urology, First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China
关键词:
膀胱肿瘤 整合素连接激酶免疫组织化学逆转录聚合酶链反应
Keywords:
bladder neoplasms integrin-linked kinase immunohistochemistry reverse transcriptase polymerase chain reaction
分类号:
R345; R730.23; R737.14
文献标志码:
A
摘要:
目的   检测膀胱移行细胞癌(bladder transitional cell carcinoma, BTCC)和癌旁正常膀胱组织中整合素连接激酶(ILK)蛋白和mRNA的表达,探讨其在BTCC发生、发展中的作用。   方法   利用免疫组织化学S-P法检测56例BTCC和30例癌旁正常膀胱组织中ILK蛋白的表达,同时运用逆转录聚合酶链反应法检测这些组织标本中ILK mRNA的表达,并与临床病理资料进行相关性分析。   结果   56例BTCC中,ILK蛋白阳性表达率为53.6%(30/56),ILK mRNA阳性表达率为58.9%(33/56);而30例癌旁正常膀胱组织中ILK蛋白阳性表达率为10%(3/30),ILK mRNA表达阳性率为13.3%(4/30), ILK mRNA 和蛋白在癌组织和癌旁正常膀胱组织表达有显著差异(P<0.01)。临床病理资料的相关性分析显示,BTCC中,ILK mRNA和蛋白的表达分布与病理分级有相关性(P<0.05),但与临床分期无相关性(P>0.05)。   结论   ILK基因转录和蛋白表达可能参与BTCC的发生过程,ILK可能成为早期发现BTCC的一个肿瘤标志物和治疗BTCC的新靶点。
Abstract:
Objective   To investigate the roles of integrin-linked kinase (ILK) protein and mRNA in the development of human bladder transitional cell carcinoma (BTCC).     Methods   The expressions of ILK protein and ILK mRNA in specimens from 56 cases of human BTCC and from 30 cases of adjacent normal bladder were detected by immunohistochemistry S-P method and reverse transcription polymerase chain reaction (RT-PCR), respectively. The correlation of the expressions of ILK protein and mRNA to the clinicopathological parameters of human BTCC was analyzed statistically.     Results   The positive rates of ILK protein and mRNA in malignant specimens were 53.6% (30/56) and 58.9% (33/56), respectively, while those in the adjacent normal bladder tissue were 10% (3/30) and 13.3% (4/30), respectively. Significant difference was found in the expressions of ILK protein and mRNA between BTCC and adjacent normal bladder specimens (P<0.01). Further statistical analysis showed that the ILK protein and mRNA expressions were correlated to the pathological grades of BTCC (P<0.05), but not to the clinical stage of BTCC (P>0.05).     Conclusion   ILK gene transcription and protein expression may be involved in the developmental process of BTCC. ILK might be a new marker for early diagnosis of BTCC and a new target gene for BTCC treatment.

参考文献/References:

蓝建华, 王德林, 杨宗珂, 等.膀胱移行细胞癌中整合素连接激酶的表达及意义[J]. 第三军医大学学报,2009,31(13):1243-1245.

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更新日期/Last Update: 2009-06-16