[1]王云霞,张立群,罗阳,等.漏声表面波传感器生物信号放大系统的研究[J].陆军军医大学学报(原第三军医大学学报),2009,31(11):1013-1016.
　WANG Yun-xia,ZHANG Li-qun,LUO Yang,et al.Study on biological signal amplification system of leaky surface acoustic wave sensor[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(11):1013-1016.
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漏声表面波传感器生物信号放大系统的研究(/HTML )
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陆军军医大学学报（原第三军医大学学报）[ISSN:1000-5404/CN:51-1095/R]

卷:
31卷
期数:
2009年第11期
页码:
1013-1016
栏目:
论著
出版日期:
2009-06-15

文章信息/Info

Title:
Study on biological signal amplification system of leaky surface acoustic wave sensor
作者:
王云霞; 张立群; 罗阳; 丁毅; 施建峰; 徐清华; 汪广杰; 曹亮; 陈鸣; 府伟灵
第三军医大学:西南医院检验科，大坪医院野战外科研究所检验科;中国电子科技集团公司第二十六研究所五室
Author(s):
WANG Yun-xia;  ZHANG Li-qun;  LUO Yang;  DING Yi;  SHI Jian-feng;  XU Qing-hua;  WANG Guang-jie;  CAO Liang;  CHEN Ming;  FU Wei-ling
Department of Laboratory Medicine, Southwest Hospital, Third Military Medical University, Chongqing 400038; 26th Institute of China Electronic Technology Group Corporation, Chongqing 400060; Department of Laboratory Medicine, Daping Hospital, Third Military Medical University, Chongqing 400042, China
关键词:
漏声表面波; 传感器; 肽核酸; RecA蛋白; 灵敏度
Keywords:
leaky surface acoustic wave;  sensor;  peptide nucleic acid;  RecA protein;  sensitivity
分类号:
R318.04;R394-33;R446
文献标志码:
A
摘要:
目的   研究利用“RecA蛋白-互补单链DNA”探针与bis-PNA/dsDNA复合物相结合，作为生物信号放大系统提高传感器检测灵敏度的可行性。   方法   漏声表面波传感器检测通道金膜表面先固定针对人乳头瘤病毒(HPV)的bis-PNA探针，加入HPV基因组DNA与之完全反应后，再加入不同浓度的“RecA蛋白-互补单链DNA”探针，在反应过程中加入ATPγS提供能量，分别探索优化RecA蛋白及ATPγS的最佳反应浓度。   结果   当RecA蛋白浓度为45 μg/ml，所引起的相位变化值为(11.74±1.03)°，显著高于其他浓度组（P<0.01）；在最佳RecA蛋白浓度下，当ATPγS浓度为2.5 mmol/L时，所引起的相位变化值为(10.71±0.73)°，显著高于其他ATPγS浓度组（P<0.01）。   结论   “RecA蛋白-互补单链DNA”探针复合体与bis-PNA/dsDNA复合物相结合可以有效提高传感器的检测灵敏度，并明显缩短检测时间。
Abstract:
Objective   To study the feasibility of combination of  “RecA protein-complementary single strand DNA” probe and bis-PNA/dsDNA complexes as the biological signal amplification system for the improvement of the sensitivity of leaky surface acoustic wave bis-peptide nucleic acid biosensor.     Methods   Bis-PNA probe for detecting human papilloma virus (HPV) was immobilized on the surface of gold membrane of the detection channel. After the probe was hybridized with the corresponding target DNA completely, different concentrations of “RecA protein-complementary single strand DNA probe” were added to react with bis-PNA/dsDNA complexes.     Results   The phase shift value was (11.74±1.03) deg at the concentration of 45 μg/ml of RecA protein, significantly different as compared with other concentrations (P<0.01). At the optimal concentration of RecA protein, the phase shift value was (10.71±0.73) deg at the concentration of 2.5 mmol/L of ATPγS, significantly different as compared with other concentrations (P<0.01).     Conclusion   Combination of “RecA protein-complementary single strand DNA probe” and bis-PNA/dsDNA complexes contributes to effective improvement of the sensitivity and significantly shortened detection time.

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更新日期/Last Update: 2009-05-21