[1]张泽华,罗飞,张劲松,等.冻干同种异体髂骨作为支架材料的性能研究[J].第三军医大学学报,2009,31(11):1069-1072.
 ZHANG Ze-hua,LUO Fei,ZHANG Jing-song,et al.Study on properties of freeze-dried iliac crest bone allograft as scaffold material[J].J Third Mil Med Univ,2009,31(11):1069-1072.
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冻干同种异体髂骨作为支架材料的性能研究(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
31卷
期数:
2009年第11期
页码:
1069-1072
栏目:
论著
出版日期:
2009-06-15

文章信息/Info

Title:
Study on properties of freeze-dried iliac crest bone allograft as scaffold material
作者:
张泽华罗飞张劲松曾玲许建中陈卫军
第三军医大学:西南医院骨科,全军矫形外科中心,基础医学部人体解剖学教研室,重庆市生物力学重点实验室
Author(s):
ZHANG Ze-hua LUO Fei ZHANG Jing-song ZENG LingXU Jian-zhongCHEN Wei-jun
Department of Orthopaedics,  Southwest Hospital,Biomechanics Section Under the Ministry of  Education’s Key Laboratory of Biomechanics and Tissue Engineering, Third Military Medical University, Chongqing 400038, China
关键词:
冻干同种异体骨带三面皮质骨髂骨生物力学性能
Keywords:
freeze-dried bone allograft tricortical iliac crest biomechanical property
分类号:
R318.01;R318.08;R322.71
文献标志码:
A
摘要:
目的   研究冻干同种异体髂骨块支架材料的微观结构及生物力学特点。   方法   参考美国组织库协会标准,进行髂骨块采取、脱细胞、脱脂、脱蛋白、消毒、保存。分别将纤维蛋白胶、利福平负压灌注入骨块孔隙内;大体观察冻干同种异体髂骨块、载利福平髂骨块的结构特征,扫描电镜观察孔径、孔隙连通、利福平晶体分布情况,液体置换法测定孔隙率和密度,弯曲试验、扭转试验、压缩试验评价其力学性能。   结果   冻干同种异体髂骨支架材料呈乳白色,为三面薄层皮质骨及中间松质骨组成的“夹心饼结构”,具有骨组织天然三维网状孔隙,孔隙内洁净,相互连通,利福平晶体被纤维蛋白胶均匀地固定于骨块表面和内部;冻干同种异体髂骨块密度(0.35±0.03)g/ml,孔隙率(67.23±9.12)%,孔径(395.36±37.02)μm,抗扭强度(5.96±1.53)MPa,抗压强度(9.94±0.73)MPa,弹性模量(376.14±27.32)MPa,抗弯强度(15.70±2.35 )MPa。   结论   冻干髂骨块具有天然生理性孔隙,生物力学性能良好,具有广阔的开发前景。
Abstract:
Objective   To study the microstructure and biomechanical properties of freeze-dried iliac crest bone allograft with regard to its potential applications as bone substitute, scaffold for bone tissue engineering and in drug delivery system.    Methods   Procurement, processing, sterilization and conservation of the iliac crest allograft scaffold were all performed according to the guidelines of the American Association of Tissue Banks. The graft segments were impregnated with Fibrin glue and rifampicin using negative pressure technique. Its physical characteristics were observed including colour, texture and porosities.The pore diameter  and microstructure were investigated with scanning electron microscope.Its porosity and density were measured by liquid displacement. Biomechanical tests were conducted in axial compression, lateral bending and rotation modes.    Results   The freeze-dried iliac crest bone allograft was ivory white, clean and porous, presenting sandwich structure in tricortical and cancellous bone. The rifampicin crystal was cemented in the pore by fibrin. Its mean density ,porosity and pore diameter was (0.35±0.03)g/ml, (67.23±9.12)%, (395.36±37.02)μm, respectively. The mean anti-rotation strength, compressive strength, elastic modulus, buckling strength was (5.96±1.53),(9.94±0.73),(376.14±27.32),(15.70±2.35)MPa, respectively.    Conclusion   The freeze-dried iliac crest bone allograft is a natural porous bone substitute with satisfying biomechanical strength, showing a great potential for further exploration.
更新日期/Last Update: 2009-05-21