[1]梁培禾,张克勤,聂志林,等.Flk-1265-2493与C3d3基因融合质粒诱导小鼠免疫应答的初步研究[J].第三军医大学学报,2009,31(13):1239-1342.
 LIANG Pei-he,ZHANG Ke-qin,NIE Zhi-lin,et al.Immunological effect of a DNA vaccine encoding genetic fusion of Flk-1265-2493 and three copies of C3d[J].J Third Mil Med Univ,2009,31(13):1239-1342.
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Flk-1265-2493与C3d3基因融合质粒诱导小鼠免疫应答的初步研究
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
31卷
期数:
2009年第13期
页码:
1239-1342
栏目:
专题报道
出版日期:
2009-07-15

文章信息/Info

Title:
Immunological effect of a DNA vaccine encoding genetic fusion of Flk-1265-2493 and three copies of C3d
作者:
梁培禾张克勤聂志林许桂莲李彦锋叶锦靳风烁
第三军医大学:大坪医院野战外科研究所泌尿外科,基础医学部全军免疫学研究所
Author(s):
LIANG Pei-he ZHANG Ke-qin NIE Zhi-lin XU Gui-lian LI Yan-feng  YE Jin JIN Feng-shuo
Department of Urology, Daping Hospital, Institute of Surgery Research, Third Military Medical University, Chongqing 400042, Institute of Immunology, College of Basic Medical Sciences, Third Military Medical University, Chongqing 400038, China
关键词:
血管新生DNA疫苗Flk-1C3d
Keywords:
angiogenesis DNA vaccine Flk-1 C3d
分类号:
R392-33;R392.2;R392.7
文献标志码:
A
摘要:
目的   对pSG.SS.Flk-1265-2493.C3d3.YL及pSG.SS.Flk-1265-2493.YL真核表达质粒的免疫效应进行初步研究。    方法   构建pSG.SS.Flk-1265-2493.C3d3.YL及pSG.SS.Flk-1265-2493.YL真核表达质粒后,48只BALB/c小鼠随机均分为疫苗组、疫苗对照组、质粒对照组、空白对照组(12只/组),分别肌肉注射接种此2种质粒、pSG.SS.YL质粒和PBS。免疫后动物:①每组6只取脾脏分离细胞,MTT法测定经Flk-1蛋白刺激后的增殖效应,乳酸脱氢酶释放法测定对培养小鼠肺微血管内皮细胞的杀伤作用;②其余6只动物于不同时间点断尾取血分离血清,ELISA法测定抗Flk-1抗体水平的动态变化。   结果   经Flk-1蛋白刺激,疫苗组小鼠脾淋巴细胞的增殖效应明显较强,刺激指数显著高于其他3组(P均<0.01),对靶细胞(MVECs)的杀伤活性在不同效/靶比时均显著高于其他3组(P均<0.01)。疫苗组动物血清抗Flk-1抗体比对照疫苗组抗体阳性反应出现早2周,二者峰值效价均出现在5周(血清稀释度:1∶3840 vs 1∶60),前者12周时仍保持较高水平(1∶960),后者10周时抗体阳性反应已消失。   结论   C3d3的连接有效增强了Flk-1265-2493的免疫原性。
Abstract:
Objective   To evaluate the immunological effect of eukaryotic expression plasmids pSG.SS.Flk-1265-2493.C3d3.YL and pSG.SS.Flk-1265-2493.YL.    Methods   After construction of eukaryotic expression plasmids pSG.SS.Flk-1265-2493.C3d3.YL and pSG.SS.Flk-1265-2493.YL, BALB/c female mice (n=48) were separated into four groups (n=12 per group) and were inoculated by intramuscular injection of the two plasmids, pSG.SS.YL plasmid (plasmid control) and PBS (blank control), respectively. After inoculation, proliferation of spleen lymphocytes stimulated with Flk-1 protein was studied using MTT method and the specific cytotoxicity T lymphocyte (CTL) effect on target cells (microvascular endothelial cells, MVECs) was assayed with lactate dehydrogenase-release method. Then anti-Flk-1 antibody titers in the immune sera were assayed with indirect enzyme-linked immunosordent assay (ELISA).     Results   After stimulation with Flk-l antigen, the proliferation and specific CTL activities of spleen lymphocytes in mice immunized with pSG.SS.Flk-1265-2493.C3d3.YL were obviously stronger than those in other three groups. Although both plasmids could elicit Flk-1 specific antibody, in pSG.SS.Flk-1265-2493.C3d3.YL immunized mice, the antibody presented 2 weeks earlier, and the titer was significantly higher than that in mice immunized with pSG.SS.Flk-1265-2493.YL.     Conclusion   The results show that the C3d3 fusion strategy can enhance the immunogenicity of Flk-1 significantly

参考文献/References:

梁培禾, 张克勤, 聂志林, 等.Flk-1265-2493与C3d3基因融合质粒诱导小鼠免疫应答的初步研究[J]. 第三军医大学学报,2009,31(13):1239-1242.

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更新日期/Last Update: 2009-06-16