[1]李戈,王明.应用RNAi技术沉默survivin基因对肾癌786-O细胞的影响[J].第三军医大学学报,2009,31(24):2444-2447.
 LI Ge,WANG Ming.Silencing survivin gene with RNAi inhibits proliferation of human renal cancer cell line 786-O[J].J Third Mil Med Univ,2009,31(24):2444-2447.
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应用RNAi技术沉默survivin基因对肾癌786-O细胞的影响(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
31卷
期数:
2009年第24期
页码:
2444-2447
栏目:
论著
出版日期:
2009-12-30

文章信息/Info

Title:
Silencing survivin gene with RNAi inhibits proliferation of human renal cancer cell line 786-O
作者:
李戈王明
重庆医科大学附属第一医院泌尿外科
Author(s):
LI Ge WANG Ming
Department of Urinary Surgery, First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China
关键词:
RNA干扰survivin肾癌基因治疗
Keywords:
RNA interference surviving renal cancer gene therapy
分类号:
R394-33;R73-362;R737.11
文献标志码:
A
摘要:
目的    运用RNA干扰(RNA interference,RNAi)技术特异性阻断肾癌786-O细胞系中survivin基因的表达,并研究survivin沉默后对786-O细胞的影响。    方法    用真核转录载体pcDNATM6.2-GW/EmGFPmiR构建针对survivin基因的干扰载体,用脂质体法转染肾癌786-O细胞,通过RT-PCR、免疫组化分别检测survivin基因的mRNA和蛋白水平的表达,MTT法检测细胞生长的影响。    结果    干扰载体有效地阻断了786-O细胞中survivin基因在mRNA与蛋白水平上的表达,细胞活性受到抑制。    结论    RNAi技术沉默survivin基因可以下调肾癌786-O细胞survivin基因的表达,抑制细胞的活性。
Abstract:
Objective    To explore the inhibitory effects of RNAi targeting survivin to suppress the expression of survivin and to induce the apoptosis of human renal cancer cell line 786-O.     Methods    The target gene fragments were cloned into pcDNATM 6.2-GW/EmGFPmiR vectors, and then the recombinant eukaryotic expression vectors were transfected into 786-O cells with liposomes. The expression changes in survivin mRNA and protein were detected by RT-PCR and immunohistochemical assay, and the proliferation inhibitory rate (IR) of 786-O cells was detected by M’TT assay.     Results    The mRNA and protein expressions of survivin in the siRNA group were significantly inhibited by the eukaryotic expression vectors, and it also inhibited significantly the growth and proliferation of 786-O cells.     Conclusion    Silencing survivin gene by the RNAi technique can not only effectively decrease the expressions of survivin gene and protein, but also inhibit the cell proliferation in 786-O cells. This may provide the experimental evidence for the survivin gene as a target of gene therapy in renal cancer.

参考文献/References:

李戈,王明.应用RNAi技术沉默survivin基因对肾癌786-O细胞的影响[J]. 第三军医大学学报,2009,31(24):2444-2447.

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更新日期/Last Update: 2009-12-07