[1]赵永亮,余佩武,雷晓,等.JAK2/STAT3途径在IL-6抑制LPS诱导的树突状细胞分化成熟中的作用[J].第三军医大学学报,2009,31(11):1063-1065.
 ZHAO Yong-liang,YU Pei-wu,LEI Xiao,et al.IL-6 suppresses LPS-induced DCs maturation via JAK2/STAT3 pathway[J].J Third Mil Med Univ,2009,31(11):1063-1065.
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JAK2/STAT3途径在IL-6抑制LPS诱导的树突状细胞分化成熟中的作用(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
31卷
期数:
2009年第11期
页码:
1063-1065
栏目:
论著
出版日期:
2009-06-15

文章信息/Info

Title:
IL-6 suppresses LPS-induced DCs maturation via JAK2/STAT3 pathway
作者:
赵永亮余佩武雷晓刘伟饶芸
第三军医大学西南医院全军普通外科中心,微创胃肠外科中心
Author(s):
ZHAO Yong-liang YU Pei-wu LEI Xiao LIU Wei RAO Yun
Department of General Surgery, Southwest Hospital, Third Military Medical University, Chongqing 400038, China
关键词:
髓源性树突状细胞白细胞介素-6LPSJAK2/STAT3信号途径
Keywords:
bone marrow derived dendritic cells IL-6 LPS JAK2/STAT3
分类号:
R338.12;R392.11;R392.5
文献标志码:
A
摘要:
目的   观察JAK2/STAT3信号途径在IL-6抑制LPS诱导的小鼠髓源性树突状细胞(bone marrow-derived dendritic cells, BMDCs)分化成熟中的作用。   方法   分离小鼠骨髓细胞,采用黏附法结合GM-CSF和IL-4刺激法在体外培养BMDCs;用相差倒置显微镜观察BMDCs的形态特点;用LPS诱导DCs成熟;采用流式细胞术观察IL-6处理与否,LPS诱导的BMDCs在表型上的变化;Western blot检测BMDCs细胞质p-JAK2、总STAT3和p-STAT3的表达水平。   结果   形态观察和FCM分析的结果表明:体外成功培养了纯度较高的高表达CD11c的BMDCs,LPS能明显诱导BMDCs表面MHC-Ⅱ类分子和共刺激分子CD80、CD86、CD40的表达上调;而经过IL-6处理后,LPS诱导BMDCs表面MHC-Ⅱ类分子、CD80、CD86、CD40表达上调的能力被显著削弱(P<0.01)。Western blot结果显示,与单独用LPS处理的BMDCs相比,经IL-6处理后的BMDC中p-JAK2、p-STAT3表达随作用时间增加而明显上调,提示IL-6促进JAK2/STAT3信号途径活化。    结论   JAK2/STAT3信号途径参与了IL-6抑制LPS诱导的BMDCs成熟过程。
Abstract:
Objective   To investigate the role of JAK2/STAT3 signaling pathway in IL-6-induced suppression during the maturation of mouse bone marrow-derived dendritic cells (BMDCs) after LPS treatment.    Methods   To obtain immature BMDCs, mouse bone marrow cells were isolated and cultured in complete culture medium containing GM-CSF and IL-4 after removing the nonadherent cells. The cell morphology was observed by using a phase contrast inverted microscope. LPS was added into the culture medium of BMDCs to induce the cell maturation. The expression of costimulators and MHC class   Ⅱ    molecules on the surface of LPS-treated BMDCs with or without IL-6 treatment was analyzed by flow cytometry. The expression of p-JAK2, STAT3 and p-STAT3 were detected by Western blot analysis.    Results   DCs were obtained from mouse bone marrow with normal morphology and high purity. Compared with BMDCs incubated with LPS only, LPS-treated DCs cultured in the presence of IL-6 expressed much lower levels of CD40, CD80, CD86 and MHC class  Ⅱ    molecules. The expression level of cytoplasmic p-JAK2 and p-STAT3 in BMDCs treated with both LPS and IL-6 were much higher than in BMDCs treated with LPS only.    Conclusion   JAK2/STAT3 pathway may be involved in the suppression of LPS-induced DCs maturation by IL-6.

参考文献/References:

赵永亮, 余佩武, 雷晓, 等. JAK2/STAT3途径在IL-6抑制LPS诱导的树突状细胞分化成熟中的作用[J]. 第三军医大学学报,2009,31(11):1063-1065.

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更新日期/Last Update: 2009-05-21