[1]王强,宋波.p38MAPK在体外培养脊髓星形胶质细胞P物质刺激活化中的作用[J].陆军军医大学学报(原第三军医大学学报),2009,31(08):689-693.
 WANG Qiang,SONG Bo.Role of p38MAPK in substance P-induced activation of spinal cord astrocytes in vitro[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(08):689-693.
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p38MAPK在体外培养脊髓星形胶质细胞P物质刺激活化中的作用(/HTML )
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陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
31卷
期数:
2009年第08期
页码:
689-693
栏目:
论著
出版日期:
2009-04-30

文章信息/Info

Title:
Role of p38MAPK in substance P-induced activation of spinal cord astrocytes in vitro
作者:
王强宋波
第三军医大学西南医院全军泌尿外科研究所
Author(s):
WANG Qiang SONG Bo
Urinary Surgery Research Institute, Southwest Hospital, Third Military Medical University, Chongqing 400038, China
关键词:
脊髓星形胶质细胞活化p38MAPK
Keywords:
spinal cord astrocytes activation p38MAPK
分类号:
R322.81;R329.25;R392.13
文献标志码:
A
摘要:
目的    观察体外培养脊髓星形胶质细胞P物质刺激活化过程中p38MAPK活性、TNF-α、NO水平及NOS活性的变化,探讨p38MAPK活性变化与脊髓星形胶质细胞活化的关系。    方法    分离培养的SPF大鼠脊髓星形胶质细胞设正常对照组(正常组)、SP刺激组(SP组,10-7mol/L)、SB203580阻断p38MAPK组(SB组,10 μmol/L)和SP刺激+SB203580阻断p38MAPK组(SP+SB组)。WB法、RT-PCR法、ELISA法检测1,3,12,24 h和36 h时p38MAPK、p-p38(磷酸化p38MAPK)含量及GFAP mRNA、TNF-α、NO水平和NOS活性的变化。    结果    脊髓星形胶质细胞GFAP 阳性表达率大于95%。SP组脊髓星形胶质细胞总p38MAPK水平无显著变化,1 h后p-p38开始升高,3 h后GFAP mRNA水平显著增高,同时NO、NOS和TNF-a水平显著增高。用SB203580阻断p38MAPK通路后,SP+SB组较SP组p-p38、GFAP mRNA、NO、NOS、TNF-a水平显著降低。SB组总p38MAPK、p-p38、GFAP mRNA、NO、NOS、TNF-a水平无显著变化。    结论    p38MAPK信号通路参与了脊髓星形胶质细胞P物质刺激活化过程,阻断p38MAPK信号通路可有效降低炎性因子水平。
Abstract:
Objective    To investigate the relationship between p38MAPK and astrocytes activation by detecting the changes of p38MAPK, TNF-α, NO and NOS in the substance P-induced activation of spinal cord astrocytes in vitro.      Methods    Astrocytes, cultured from spinal cord of SPF rat and identified by immunofluorescence method with GFAP antibody, were grouped into the control group, the SP stimulus group (SP group) and the SB203580 interrupt p38MAPK group (interrupt group) in which SP (10-7 mol/L) and SB203580 (10 μmol/L) were added to the supernatant for 12 h. The WB method, RT-PCR and ELISA were used to determine the changes of p38MAPK, phosphorylated p38MAPK, GFAP mRNA, TNF-α, NO and NOS in the astrocytes or supernatant at 1, 3, 12, 24 and 36 h respectively.     Results    The GFAP positive rate of the cultured cells was higher than 95%. p38MAPK level in the SP group did not have any change, whereas p-p38 level increased significantly after 1 h and GFAP mRNA, NO, NOS and TNF-α level increased after 3 h. When p38MAPK pathway was inhibited by SB203580 in the SP+SB group, GFAP mRNA, NO, NOS and TNF-α were significantly reduced compared with those in the SP group. The level of p38MAPK, p-p38, GFAP mRNA, NO, NOS and TNF-α in the SB group did not have changes compared with those in the normal group.     Conclusion    These results indicate that p38MAPK signal pathway contributes to substance P induced activation of spinal cord astrocytes according to inflammatory factors attenuation after p38MAPK signal pathway interruption by SB203580.

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更新日期/Last Update: 2009-04-15