[1]张慧宇,向学熔,范小平.DEX和rhBMP2对体外培养人牙髓细胞碱性磷酸酶活性的影响[J].第三军医大学学报,2009,31(13):1264-1267.
 Hui-yu,XIANG Xue-rong,FAN Xiao-ping.Effects of DEX and rhBMP2 on alkaline phosphatase activity of human dental pulp cells in vitro[J].J Third Mil Med Univ,2009,31(13):1264-1267.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
31卷
期数:
2009年第13期
页码:
1264-1267
栏目:
论著
出版日期:
2009-07-15

文章信息/Info

Title:
Effects of DEX and rhBMP2 on alkaline phosphatase activity of human dental pulp cells in vitro
作者:
张慧宇 向学熔 范小平
重庆医科大学附属口腔医院: 牙周黏膜科,正畸科
Author(s):
Hui-yu XIANG Xue-rong FAN Xiao-ping
Department of Paradental Mucosal Diseases, Department of Orthodontics,  Affiliated Hospital of Stomatology, Chongqing Medical University, Chongqing 400015, China
关键词:
地塞米松重组人骨形态发生蛋白2人牙髓细胞碱性磷酸酶
Keywords:
dexamethasone recombinated bone morphogenetic protein-2 human dental pulp cells alkaline phosphatase
分类号:
R345; R446.19; R78
文献标志码:
A
摘要:
目的   探讨不同浓度地塞米松(Dexamethasone,DEX)、重组人骨形态发生蛋白2(recombinant human bone morphogenetic protein 2, rhBMP2)及两者联合应用对体外培养的人牙髓细胞(human dental pulp cells,HDPCs)碱性磷酸酶(Alkaline Phosphatase,ALP)活性的影响。   方法   组织块法培养HDPCs并进行鉴定;采用酶动力学的方法,观察DEX、rhBMP2 及二者联合应用对HDPCs 的ALP活性的影响。   结果   单独应用DEX时ALP活性明显增高,且在生理浓度范围内当浓度为0.01 nmol/ml时,对HDPCs的ALP活性达到最大的刺激作用,rhBMP2对HDPCs的ALP活性呈浓度依赖性增强;当联合应用DEX和rhBMP2,ALP活性比单独应用相应浓度的rhBMP2明显增高。   结论   DEX、rhBMP2对HDPCs的ALP活性均有增强作用,同时二者对HDPCs的ALP活性有显著的功能放大性协同增强作用。
Abstract:
Objective   To study the effects of dexamethasone (DEX), recombinant bone morphogenetic protein-2 (rhBMP2), and combined application of rhBMP2 and DEX on alkaline phosphatase (ALP) activity of human dental pulp cells (HDPCs) in vitro.     Methods   HDPCs were cultured by tissue block method and identified. Effects of DEX, rhBMP2, and combined application of both on ALP activity of HDPCs were determined by a modified enzyme dynamical method.     Results   DEX could enhance ALP activity, reaching the peak value at the concentration of 0.01 nmol/ml. rhBMP2 could enhance ALP activity in a dose-dependent manner. ALP activity was significantly higher under the condition of combined application of DEX and rhBMP2 than single application of DEX or rhBMP2 only.     Conclusion   Both DEX and rhBMP2 can enhance ALP activity of HDPCs. However, combined application of DEX and rhBMP2 can greatly enhance ALP activity of HDPCs.

参考文献/References:

张慧宇, 向学熔, 范小平.DEX和rhBMP2对体外培养人牙髓细胞碱性磷酸酶活性的影响[J]. 第三军医大学学报,2009,31(13):1264-1267.

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更新日期/Last Update: 2009-06-16