[1]刘文斌,刘晋袆,周紫垣,等.大鼠肺鳞癌癌变过程中细胞周期调控蛋白的动态表达[J].第三军医大学学报,2009,31(07):577-580.
 LIU Wen-bin,LIU Jin-yi,ZHOU Zi-yuan,et al.Dynamic expression of cell cycle regulation related proteins during carcinogenesis of lung squamous cell carcinoma in rats[J].J Third Mil Med Univ,2009,31(07):577-580.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
31卷
期数:
2009年第07期
页码:
577-580
栏目:
论著
出版日期:
2009-04-15

文章信息/Info

Title:
Dynamic expression of cell cycle regulation related proteins during carcinogenesis of lung squamous cell carcinoma in rats
作者:
刘文斌刘晋袆周紫垣周燕虹敖琳崔志鸿曹佳
第三军医大学军事预防医学院军事毒理学教研室
Author(s):
LIU Wen-bin LIU Jin-yi ZHOU Zi-yuan ZHOU Yan-hong AO Lin CUI Zhi-hong CAO Jia
Department of Military Hygienic Toxicology, College of Military Preventive Medicine, Third Military Medical University, Chongqing 400038, China
关键词:
肺肿瘤癌变P16P27P53
Keywords:
lung cancer carcinogenesis P16 P27 P53
分类号:
R394.3;R730.23;R734.2
文献标志码:
A
摘要:
目的    探讨化合物诱导的大鼠肺鳞癌癌变各阶段细胞周期调控蛋白P16、P27和P53水平动态变化趋势、相互关系及其在癌变过程中的作用。    方法    3-甲基胆蒽(MCA)及二乙基亚硝胺(DEN)碘油溶液诱发Wistar大鼠肺鳞癌。采用免疫组化S-P法检测P16、P27和P53蛋白在大鼠肺鳞癌癌变各阶段的表达水平,采用Western blot检测鳞癌、癌旁和正常组织中各蛋白表达水平。    结果    共获取癌变各阶段标本164例,其中正常支气管上皮20例,增生25例,鳞状化生27例,不典型增生37例,原位癌30例,浸润癌25例。P16和P27蛋白表达的阳性率随癌变进展而降低,P53蛋白表达的阳性率随癌变进展而升高。P16与P27蛋白表达呈正相关(P<0.01),P16和P27蛋白表达与P53蛋白表达呈负相关(P<0.05)。鳞癌组织中P16与P27蛋白低表达,P53蛋白高表达,与正常组织或癌旁组织有显著性差异(P<0.01)。    结论    在癌变过程中细胞周期调控蛋白P16和P27 表达下调,P53蛋白表达逐渐增强,可能与大鼠肺鳞癌的发生、发展密切相关。
Abstract:
Objective    To investigate dynamic expressions and correlation of cell cycle regulation related proteins, P16, P27 and P53 during the carcinogenesis of chemical-induced lung squamous cell carcinoma in Wistar rats.     Methods    Squamous cell carcinoma of lung was induced by left intra-bronchial instillating with 3-methylcholanthrene (MCA) and diethyinitrosamine (DEN) in iodized oil to 80 Wistar rats. Another 10 Wistar rats receiving intrabronchial instillation of iodized oil served as control. In 15, 35, 55, 65 and 75 d after the instillation, 16 rats from experimental group and 2 from the control were sacrificed for HE and SP staining, and Western blotting analysis. SP method was used to examine the expressions of P16, P27 and P53 proteins. Normal tissue, tumor tissue and corresponding adjacent noncancerous tissues were examined by Western blot analysis for the protein expressions of P16, P27 and P53.     Results    A total of 154 specimens at various pathological phases during carcinogenesis were obtained, including 25 hyperplasia, 27 squamous metaplasia, 37 dysplasia, 30 carcinoma in situ and 25 infiltrating carcinoma. The positive rates of P16 and P27 proteins were decreased and the positive rate of P53 protein was increased obviously when the carcinoma developed. The expression of P16 was positively correlated with P27 (P<0.01). The expression of P53 was negatively correlated with P16 and P27 (P<0.05). The downregulated P16 and P27 expressions, and P53 overexpression were frequently found in lung carcinoma. There was very significant statistical difference between carcinoma and corresponding adjacent noncancerous tissue or normal tissue (P<0.01).     Conclusion    The decreased expressions of P16, P27 and increased expression of P53 in the process of carcinogenesis may be associated with the incidence and development of lung squamous cell carcinoma in rats.

参考文献/References:

刘文斌,刘晋袆,周紫垣,等. 大鼠肺鳞癌癌变过程中细胞周期调控蛋白的动态表达[J]. 第三军医大学学报,2009,31(7):577-580.

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更新日期/Last Update: 2009-04-15