[1]钟玲,邱妍川,尚京川.人血浆中屈昔多巴的测定及药代动力学研究[J].陆军军医大学学报(原第三军医大学学报),2009,31(11):1112-1114.
 ZHONG Ling,QIU Yan-chuan,SHANG Jing-chuan.Determination of droxidopa in human plasma and pharmacokinetic features[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(11):1112-1114.
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人血浆中屈昔多巴的测定及药代动力学研究(/HTML )
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陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
31卷
期数:
2009年第11期
页码:
1112-1114
栏目:
论著
出版日期:
2009-06-15

文章信息/Info

Title:
Determination of droxidopa in human plasma and pharmacokinetic features
作者:
钟玲 邱妍川 尚京川
重庆医科大学药学院药物分析教研室;重庆医药高等专科学校药剂教研室
Author(s):
ZHONG Ling QIU Yan-chuan SHANG Jing-chuan
Department of Drug Analysis, School of Pharmaceutical Science, Chongqing Medical University, Chongqing 400016; Department of Pharmaceutics, Chongqing Medical and Pharmaceutical College, Chongqing 400030, China
关键词:
屈昔多巴血药浓度药代动力学高效液相色谱法
Keywords:
droxidopa plasma concentration pharmacokinetics high performance liquid chromatography
分类号:
R927.2;R969.1;R977.1
文献标志码:
A
摘要:
目的   建立测定人血浆中屈昔多巴的方法,并应用于药代动力学研究。   方法   12例健康受试者单剂量口服屈昔多巴胶囊200 mg后,血样经高氯酸沉淀蛋白,通过HPLC-荧光检测法测定屈昔多巴的浓度,并运用3P87分析软件计算其药代动力学参数。   结果   屈昔多巴测定的线性范围为0.025~2 μg/ml,其主要的药动学参数T1/2108.50±30.78)min,曲线下峰面积(AUC) (185.74±26.41)μg·ml-1·min-1,消除率(CL)(1.08±0.08)ml/min,Tmax(99.90±10.05)min,Cmax(0.74±0.08)μg/ml,服药10 h后屈昔多巴几乎从血液中完全消除。   结论   本方法灵敏度高,重现性好,操作简便,可用于血浆样品中屈昔多巴的定性定量检测;屈昔多巴在体内的药代动力学过程符合二室模型。
Abstract:
Objective   To propose a method for the determination of droxidopa concentration in human plasma by HPLC method for the study of the pharmacokinetics of droxidopa.    Methods   After a single oral dose of 200 mg droxidopa capsule was administered to 12 healthy Chinese male volunteers, we used perchloric acid to precipitate proteinum and then determined the droxidopa concentration in plasma by HPLC. The pharmacokinetic parameters of droxidopa were calculated by 3P87 software.    Results   The linear range was 0.025-2 μg/ml. Pharmacokinetic parameters were: T1/2(108.50±30.78)min, AUC (185.74±26.41) μg·ml-1·min-1, CL (1.08±0.08)ml/min, Tmax(99.90±10.05)min, Cmax(0.74±0.08)μg/ml. At 10 h after administration, droxidopa was eliminated from the blood almost completely.    Conclusion   The method we proposed is sensitive, reproducible, and easy to operate. Pharmacokinetics of droxidopa in vivo is fitted to two-compartment model.

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更新日期/Last Update: 2009-05-21