[1]于智勇,沈维干,薛庆於,等.AdH1-siRNA-Survivin抗小鼠黑色素瘤生长和转移的研究[J].第三军医大学学报,2008,30(18):1706-1709.
 YU Zhi-yong,SHEN Wei-gan,XUE Qing-yu,et al.AdH1-siRNA-Survivin inhibits tumor growth and metastasis of mouse melanoma[J].J Third Mil Med Univ,2008,30(18):1706-1709.
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AdH1-siRNA-Survivin抗小鼠黑色素瘤生长和转移的研究(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
30卷
期数:
2008年第18期
页码:
1706-1709
栏目:
论著
出版日期:
2008-09-24

文章信息/Info

Title:
AdH1-siRNA-Survivin inhibits tumor growth and metastasis of mouse melanoma
作者:
于智勇沈维干薛庆於武辉
扬州大学医学院细胞生物学教研室;扬州教育学院生化工程系
Author(s):
YU Zhi-yong SHEN Wei-gan XUE Qing-yu WU Hui
Department of Cell Biology, Medical College of Yangzhou University, Yangzhou 225009; Department of Biochemistry Engineering, Yangzhou Educational College, Yangzhou 225009, Jiangsu, China
关键词:
RNA干扰重组腺病毒Survivin黑色素瘤
Keywords:
RNA interference recombinant adenovirus Survivin melanoma
分类号:
R394-33;R73-362;R739.5
文献标志码:
A
摘要:
目的    研究针对Survivin的腺病毒介导的siRNA对小鼠黑色素瘤生长和转移的影响。    方法    构建表达小鼠Survivin序列特异性siRNA复制缺陷型重组腺病毒(AdH1-siRNA-Survivin),利用Western blot方法检测感染AdH1-siRNA-Survivin的B16BL6细胞中Survivin蛋白的表达;采用皮下注射法建立小鼠黑色素瘤生长模型,采用瘤内或周围多点注射相应的重组腺病毒,每周1次,每次2×109 FPU,共3次。定期测量各组肿瘤体积,应用免疫组化法检测肿瘤微血管密度(MVD);采用尾静脉注射感染重组腺病毒的B16BL6细胞建立小鼠肿瘤转移模型,计数并比较各组小鼠肺表面的黑色转移结节数。    结果    AdH1-siRNA- Survivin可以显著下调B16BL6细胞Survivin蛋白的表达;与空白对照组和空载病毒组(AdH1-Empty)相比,AdH1-siRNA-Survivin注射组显著抑制小鼠肿瘤的生长,降低肿瘤的MVD数(P<0.01);感染AdH1-siRNA-Survivin的B16BL6细胞小鼠的肺转移灶数目也显著降低(P<0.01)。    结论    重组腺病毒介导的针对Survivin的siRNA技术有效地下调细胞中Survivin的表达,能减少肿瘤内微血管生成、抑制肿瘤生长和转移。
Abstract:
Objective    To evaluate the effect of adenovirus-mediated siRNA targeting Survivin on tumor growth and metastasis of mouse melanoma.     Methods    A replication-defective recombinant adenovirus AdH1-siRNA-Survivin targeting mouse Survivin was constructed using the homologous recombination technique. The expression of Survivin protein in B16BL6 cells after infection was detected by Western blotting. Mouse melanoma models were established by subcutaneous injection of B16BL6 cells. AdH1-siRNA-Survivin of 2×109 pfu every week by multi-spot intratumoral or peritumoral injection was performed to every mouse (totally three times). The tumor volume was measured every five days after tumor cell implantation, and the microvessel density (MVD) of tumor was counted by immunohistochemistry under the microscope. For the experimental metastasis assay, B16BL6 cells infected with AdH1-siRNA-Survivin were injected into the tail veins of mice, then the visible black nodules on the lung surface were counted macroscopically at 21th day after tumor cell injection.     Results    AdH1-siRNA-Survivin could obviously decrease the expression of Survivin protein in B16BL6 cells. When compared with the blank control and AdH1-empty control, the tumor growth was inhibited and MVD was less in the mice injected with AdH1-siRNA-Survivin (P<0.01), and lung nodules was significantly less, too (P<0.01).     Conclusion    The recombinant adenovirus-mediated siRNA targeting Survivin can obviously down-regulate Survivin expression in B16BL6 cells and inhibit tumor angiogenesis, growth and metastases.

参考文献/References:

于智勇,沈维干,薛庆於,等.  AdH1-siRNA-Survivin抗小鼠黑色素瘤生长和转移的研究[J]. 第三军医大学学报, 2008, 30(18):1706-1709.

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更新日期/Last Update: 2008-09-11