[1]熊加祥,白云,宋敏,等.炎性介质脂多糖、γ干扰素活化星形胶质细胞效应[J].第三军医大学学报,2008,30(22):2065-2068.
 XIONG Jia-xiang,BAI Yun,SONG Min,et al.Activation of cultured mouse astrocytes by LPS and IFN-γ[J].J Third Mil Med Univ,2008,30(22):2065-2068.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
30卷
期数:
2008年第22期
页码:
2065-2068
栏目:
论著
出版日期:
2008-11-30

文章信息/Info

Title:
Activation of cultured mouse astrocytes by LPS and IFN-γ
作者:
熊加祥白云宋敏许雪青王艳艳杨晓亚
第三军医大学基础医学部:医学遗传学教研室,生理学教研室
Author(s):
XIONG Jia-xiang BAI Yun SONG Min XU Xue-qing WANG Yan-yan YANG Xiao-ya
Department of Molecular Genetics, Department of Physiology, College of Medicine, Third Military Medical University, Chongqing 400038, China
关键词:
脂多糖γ干扰素 星形胶质细胞活化
Keywords:
LPS IFN-γ astrocytes activation
分类号:
R329.25;R338.1;R392.11
文献标志码:
A
摘要:
目的    研究炎性介质脂多糖(LPS)、γ干扰素(IFN-γ)刺激离体星形胶质细胞的效应。    方法    LPS、IFN-γ刺激离体培养纯化的小鼠星形胶质细胞,ELISA检测细胞分泌TNF-α,Griess法测定NO浓度,激光共聚焦扫描显微镜(CLSM)检测细胞内游离钙浓度([Ca2+]i)。    结果    LPS(500 ng/ml)、IFN-γ(100 U/ml)刺激星形胶质细胞24 h后, NO和TNF-α的分泌显著升高,联合应用有协同作用;延长IFN-γ作用时间,分泌量升高。CLSM检测LPS、IFN-γ刺激24 h后的星形胶质细胞[Ca2+]i的荧光值,显示荧光相对值比正常细胞明显升高;LPS、IFN-γ急性刺激正常培养细胞发现[Ca2+]i振荡上升;而作用于IFN-γ刺激24 h后的细胞,[Ca2+]i上升明显减缓。    结论    LPS、IFN-γ可以活化星形胶质细胞,表现为[Ca2+]i升高,TNF-α和NO分泌上调。
Abstract:
Objective    To investigate the effects of inflammatory chemokine LPS and/or IFN-γ on astrocytes.     Methods    After LPS and IFN-γ applied to the purified cultured mouse astrocytes, TNF-α and nitric oxide (NO) secreted from astrocytes and intracellular calcium concentration ([Ca2+]i) were detected by ELISA, Griess and confocal laser scanning microscopy, respectively.     Results    Treatment with LPS (500 ng/ml) or IFN-γ (100 U/ml) for 24 h significantly increased the concentration of TNF-α and NO. Coadministration of LPS and IFN-γ showed a synergistic effect on the astrocytes. By using Ca2+ imaging, we found that [Ca2+]i signal was significantly increased after LPS and/or IFN-γ treatment for 24 h. Also, acute treatment with LPS or IFN-γ could evoke Ca2+ oscillations. In contrast, after application of these stimuli over 24 h, the Ca2+ signal was increased more slowly.     Conclusion    Application of LPS and/or IFN-γ evoked TNF-α and NO secretion and [Ca2+]i elevation in cultured astrocytes, suggesting that astrocytes could be activated by LPS and IFN-γ.

参考文献/References:

熊加祥,白云,宋敏,等. 炎性介质脂多糖、γ干扰素活化星形胶质细胞效应[J]. 第三军医大学学报,2008,30(22):2065-2068.

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更新日期/Last Update: 2008-10-30