Claudin 3在食管腺癌和贲门腺癌的表达和意义-《第三军医大学学报》

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Title:: Expression and significance of Claudin 3 in adenocarcinoma of esophagus and cardia adenocarcinoma

Author(s):: WANG Jun; FANG Dian-chun; ZHAO Jing-jing; YANG Shi-ming; Gastroenterology Research Center, Southwest Hospital, Third Military Medical University

摘要:: 目的探讨Claudin 3在食管腺癌和贲门腺癌的表达及意义。方法分别采用RT-PCR及Western blot检测16例食管腺癌、20例食管鳞癌、15例弥漫型贲门腺癌、25例肠型贲门腺癌、15例正常食管黏膜和15例正常贲门黏膜组织中Claudin 3 mRNA及蛋白表达。结果与正常对照组和食管鳞癌、贲门弥漫型腺癌组相比，食管腺癌和贲门肠化型腺癌组织中Claudin 3 mRNA及蛋白表达显著增高(P<0.01);食管鳞癌组、贲门弥漫型癌组Claudin 3 mRNA及蛋白表达与正常对照组无明显变化（P>0.05）。结论 Claudin 3与食管腺癌和贲门肠化型腺癌发生有关。

Abstract:: Objective To investigate the expression and significance of Claudin 3 in adenocarcinoma of esophagus and gastric cardia. Methods We subjected pathologically confirmed 16 cases of esophageal adenocarcinoma (EAC), 20 cases of esophageal squamous cell carcinoma (ESC), and 40 cases of gastric cardiac adenocarcinoma (CAC, including 15 diffuse and 25 intestinal cases). Tissue samples were collected with aid of gastroscope. Fifteen samples of normal esophageal mucosa and 15 samples of normal cardiac mucosa served as control. RT-PCR and Western blotting were used respectively to detect the mRNA and protein expression of Claudin 3 in these tissue samples. Results The mRNA and protein expressions of Claudin 3 were increased in EAC and intestinal CA compared with those in normal tissues, ESC and diffuse CAC. There was no significant difference of the expression of Claudin 3 between normal group and ESC and diffuse CAC group. Conclusion Claudin 3 might be related to the development of EAC and intestinal CAC, and expected to be the tumor maker and therapeutic target of this kind of carcinoma.