[1]廖亚玲,邹全明,张卫军,等.对中国4个不同地区幽门螺杆菌菌株的多位点序列分析研究[J].第三军医大学学报,2008,30(11):1049-1051.
 LIAO Ya-ling,ZOU Quan-ming,ZHANG Wei-jun,et al.Genetic diversity and vacA allele heterogeneity in Helicobacter pylori isolates from four cities of China[J].J Third Mil Med Univ,2008,30(11):1049-1051.
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对中国4个不同地区幽门螺杆菌菌株的多位点序列分析研究(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
30卷
期数:
2008年第11期
页码:
1049-1051
栏目:
论著
出版日期:
2008-06-15

文章信息/Info

Title:
Genetic diversity and vacA allele heterogeneity in Helicobacter pylori isolates from four cities of China
作者:
廖亚玲 邹全明张卫军刘晓菲钟情
第三军医大学医学检验系临床微生物与免疫学教研室
Author(s):
LIAO Ya-ling ZOU Quan-ming ZHANG Wei-jun LIU Xiao-fei ZHONG Qing
Department of Clinical Microbiology and Immunology, College of Laboratory Medicine, Third Military Medical University, Chongqing 400038, China
关键词:
幽门螺杆菌多位点序列分析vacA基因
Keywords:
Helicobacter pylori MLST vacA
分类号:
R318.04; R372; R378
文献标志码:
A
摘要:
目的 了解国内4个不同地区(北京、上海、重庆和广州)收集的幽门螺杆菌(H.pylori)临床株的遗传背景和可能的来源。   方法  从胃肠疾病患者黏膜标本中分离培养幽门螺杆菌,应用多位点序列分析(multilocus sequence typing, MLST)对鉴定阳性的菌株进行ST分型比较,并且以PCR方法测定vacA基因型。  结果  30株临床分离的幽门螺杆菌菌株,vacAs1a阳性25株(83.3 %),vacA s1b阳性5株(16.7 %),vacA m2阳性为30株(100 %),未发现s2型菌株,其中标准株NCTC11637为vacAs1a/ m1型。共确定了28种ST型。  结论 MLST作为幽门螺杆菌分型方法,分辨率高,结果准确,易于标准化。通过信息学分析可以将MLST分型、vacA基因型和遗传背景、临床来源结合起来,适于进行分子进化学研究。
Abstract:
Objective    To evaluate the genetic diversity of 30 Helicobacter pylori isolates from the patients in four cities of China (Beijing, Shanghai, Guangzhou, Chongqing) by the multilocus sequence typing (MLST), and to find the link between the vacA genotype and sequence type (ST).     Methods    Isolates were randomly recruited from consecutive endoscopy clinics in four cities. An average of 5-6 strains per patient were isolated and eight loci selected for the MLST scheme were amplified and sequenced with primers. The vacA genotype was analyzed by PCR and 3% agarose electrophoresis.     Results    There were totally 28 ST genotypes, most of which are new ST genotypes that have never been found among the database. The most prevalent vacA genotype was s1a/m2 (83.3%), followed by s1b/m2 (13.0%). In contrast, the s1a/m1 genotype was scarcely present (0%).     Conclusion    The vacA genotype varied for each ST. Our results showed no obvious link between STs and vacA genotypes. As there are little data on human isolates due to the absence of systematic epidemiological investigations of H. pylori in medical laboratories, our research will provide useful information in understanding the epidemiology of H. pylori in China.

参考文献/References:

廖亚玲, 邹全明,张卫军,等. 对中国4个不同地区幽门螺杆菌菌株的多位点序列分析研究[J].第三军医大学学报,2008,30(11):1049-1051.

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更新日期/Last Update: 2008-06-06