[1]池余刚,钟玲,伍霞,等.原花青素对卵巢癌SKOV3细胞增殖和凋亡的影响[J].第三军医大学学报,2008,30(23):2203-2206.
 CHI Yu-gang,ZHONG Ling,WU Xia,et al.Grape seed proanthocyanidin extract inhibits proliferation and promotes apoptosis of ovary cancer cell line SKOV3[J].J Third Mil Med Univ,2008,30(23):2203-2206.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
30卷
期数:
2008年第23期
页码:
2203-2206
栏目:
论著
出版日期:
2008-12-15

文章信息/Info

Title:
Grape seed proanthocyanidin extract inhibits proliferation and promotes apoptosis of ovary cancer cell line SKOV3
作者:
池余刚 钟玲 伍霞 王勇
重庆医科大学附属第二医院妇产科
Author(s):
CHI Yu-gang ZHONG Ling WU Xia WANG Yong
Department of Gynecology and Obstetrics, Second Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China
关键词:
卵巢癌原花青素细胞增殖细胞凋亡survivinSKOV3细胞
Keywords:
ovary cancers proanthocyanidin proliferation apoptosis survivin SKOV3 cells
分类号:
R329.28; R73-361; R737.31
文献标志码:
A
摘要:
目的    探讨葡萄籽提取物原花青素对卵巢癌SKOV3细胞增殖和凋亡的影响和机制。    方法    体外培养的SKOV3细胞与25、50、100、200、400 μg/ml的葡萄籽提取物原花青素共孵育,分别在24、48、72 h时观察细胞形态学变化,MTT检测细胞增殖抑制率,流式细胞和TUNEL分析细胞凋亡,RT-PCR检测survivin在mRNA的表达情况,Western blot检测SKOV3细胞survivin在蛋白水平的表达。    结果    不同浓度(25、50、100、200、400 μg/ml)原花青素对SKOV3细胞作用48 h 的细胞增殖抑制率分别为48.67%、53.85%、67.03%、73.78%、77.39%。流式细胞和TUNEL检测经25 μg/ml原花青素处理24、48 h后SKOV3细胞凋亡率分别为31.98%、45.78%。葡萄籽原花青素还可以明显降低survivin的mRNA和蛋白水平的表达。这些作用均随原花青素浓度和作用时间的延长而增强。    结论    原花青素可能通过降低survivin的表达,在体外抑制SKOV3细胞增殖,并促进其凋亡。
Abstract:
Objective    To study the biological effects of grape seed proanthocyanidin extract (GSPE) on human ovary cancer cells and to explore the molecular mechanism.     Methods    Human ovary cancer cell line SKOV3 were co-cultured with GSPE solution of the terminal concentrations of 25, 50, 100, 200, 400 μg/ml respectively in 96-well plate. At 24, 48, 72 h after coculture, the following parameters were detected: cell growth curve, the inhibition rate of SKOV3 cells by MTT assay, DNA cycle by FCM, the apoptosis of SKOV3 by TUNEL and Annexin-V labeling method, the mRNA and protein expressions of survivin by semi-quantitative RT-PCR and Western blotting, respectively.     Results    GSPE dose-dependently inhibited the proliferation of the SKOV3 cells. Treated by GSPE, the progress of SKOV3 cells at S stage into G/M stage was inhibited. TUNEL showed that treated by 25 μg/ml GSPE for 24, 48 h, the apoptotic rates of SKOV3 cells were 31.98%, 45.78% respectively. Annexin-V showed that after incubation with 25 μg/ml GSPE for 24 h, the apoptotic rate was 14.68%. The survivin mRNA and protein expressions were both down-regulated.     Conclusion    GSPE inhibits the proliferation of malignant human ovary cancer cells and induces their apoptosis. Expression of survivin mRNA and protein may be related to cell growth inhibition and to the apoptosis mediated by GSPE in vitro.

参考文献/References:

池余刚, 钟玲, 伍霞, 等. 原花青素对卵巢癌SKOV3细胞增殖和凋亡的影响[J].  第三军医大学学报, 2008, 30(23):2203-2206.

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更新日期/Last Update: 2008-11-12