[1]鄢俊安,卢根生,李龙坤,等.豚鼠膀胱组织ICCs细胞间隙连接蛋白Connexin 43表达意义[J].第三军医大学学报,2008,30(07):561-563.
 YAN Jun-an,LU Gen-sheng,LI Long-kun,et al.Connexin 43 expression in cultured interstitial cells of Cajal from guinea pigs[J].J Third Mil Med Univ,2008,30(07):561-563.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
30卷
期数:
2008年第07期
页码:
561-563
栏目:
专题报道
出版日期:
2008-04-15

文章信息/Info

Title:
Connexin 43 expression in cultured interstitial cells of Cajal from guinea pigs
作者:
鄢俊安卢根生李龙坤宋波
第三军医大学西南医院全军泌尿外科研究所
Author(s):
YAN Jun-an LU Gen-sheng LI Long-kun SONG Bo
Urinary Surgery Research Institute, Southwest Hospital, Third Military Medical University
关键词:
Cajal间质细胞间隙连接蛋白膀胱
Keywords:
interstitial cells of Cajal gap junction protein bladder
分类号:
R322.62; R329.26; R394.2
文献标志码:
A
摘要:
目的 探讨体外培养豚鼠膀胱组织ICCs细胞(interstitial cells of Cajal,ICCs)间隙连接蛋白Connexin 43的表达。  方法 胶原酶消化法进行豚鼠膀胱组织ICCs细胞的原代培养,对照为原代培养膀胱平滑肌细胞,免疫荧光法进行两种细胞的特征性蛋白c-kit及平滑肌肌动蛋白(smooth muscle actin,SMA)染色观察,免疫荧光法及Western blot法进行培养细胞Connexin 43的检测。  结果 豚鼠膀胱ICCs细胞特征性蛋白c-kit染色阳性,SMA染色阴性。豚鼠膀胱ICCs细胞间隙连接蛋白Connexin 43表达水平较高,显著高于膀胱平滑肌细胞。  结论 豚鼠离体膀胱ICCs细胞间隙连接蛋白Connexin 43 高表达可能是其信号传递的重要物质基础。
Abstract:
Objective To explore the expression of connexin 43 in the interstitial cells of Cajal (ICCs) from guinea pig bladder in vitroMethods Bladder ICCs were primarily cultured from guinea pigs by collagenase digestion method. Primarily cultured bladder smooth muscle cells (SMCs) from guinea pig were taken as control. The expressions of c-kit and smooth muscle actin (SMA) were detected by immunofluorescent method. Immumofluorescent method and Western blotting were used to detect the expression of Connexin 43 in 2 groups of cells.  Results The expression of c-kit was positive in ICCs but negative in the control group. On the contrary, the expression of SMA was negative in ICCs but positive in the control group. Connexin 43 expression was significantly higher in ICCs than that in the control group (P<0.01).  Conclusion High expression of connexin 43 might be the important for the signal transmission in bladder ICCs of guinea pigs.

参考文献/References:

鄢俊安,卢根生,李龙坤,等. 豚鼠膀胱组织ICCs细胞间隙连接蛋白Connexin 43表达意义[J]. 第三军医大学学报,2008,30(7):561-563.

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更新日期/Last Update: 2008-04-16