[1]张丽杰,鹿刚,张引娟,等.香加皮提取物杠柳苷抑制SMMC-7721细胞Stat3信号通路诱导细胞凋亡的研究[J].陆军军医大学学报(原第三军医大学学报),2008,30(15):1448-1451.
　ZHANG Li-jie,LU Gang,ZHANG Yin-juan,et al.Periplocin of cortex periplocae inhibits Stat3 signaling and induces apoptosis in human hepatocellular carcinoma cell line SMMC-7721[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(15):1448-1451.
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香加皮提取物杠柳苷抑制SMMC-7721细胞Stat3信号通路诱导细胞凋亡的研究(/HTML )
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陆军军医大学学报（原第三军医大学学报）[ISSN:1000-5404/CN:51-1095/R]

卷:
30卷
期数:
2008年第15期
页码:
1448-1451
栏目:
论著
出版日期:
2008-08-15

文章信息/Info

Title:
Periplocin of cortex periplocae inhibits Stat3 signaling and induces apoptosis in human hepatocellular carcinoma cell line SMMC-7721
作者:
张丽杰; 鹿刚; 张引娟; 刘刚叁; 单保恩
河北医科大学：第三医院检验科，第四医院科研中心；华北制药集团新药研究开发有限责任公司
Author(s):
ZHANG Li-jie;  LU Gang;  ZHANG Yin-juan;  LIU Gang-san;  SHAN Bao-en
Clinical Laboratary, The Third Hospital, Shijiazhuang 050051; Research Center, The Fourth Hospital, Hebei Medical University, Shijiazhuang 050011; New Drug Research and Development Co., Ltd. of North China Pharmaceutical Corporation, Shijiazhuang 050015, China
关键词:
香加皮杠柳苷; 信号转导; 转录活化因子; Stat3; Western blot; 流式细胞术; SMMC-7721细胞株
Keywords:
periplocin of cortex periplocae;  signal transduction;  signal transducers and activators of transcription;  Stat3;  Western blot;  flow cytometry;  SMMC-7721 cell line
分类号:
R285.5; R730.23; R735.7
文献标志码:
A
摘要:
目的  研究香加皮提取物杠柳苷对Stat3信号通路的影响，及其诱导细胞凋亡的分子机制。 方法  MTT比色法检测人肝癌细胞SMMC-7721增殖活性，流式细胞术分析肿瘤细胞的周期变化和凋亡，Western blot检测细胞内Stat3蛋白表达，RT-PCR检测细胞Mcl-1、Survivin和XIAP mRNA的表达。 结果  杠柳苷可明显抑制SMMC-7721细胞的增殖，诱导SMMC-7721细胞凋亡，并使细胞周期停滞在G2/M期。杠柳苷作用后，细胞核内Stat3量降低，而细胞质中的量未见明显改变，细胞内Mcl-1、Survivin和XIAP基因的表达明显降低，并呈时间依赖性。 结论  香加皮提取物杠柳苷通过抑制Stat3信号转导通路和Mcl-1、Survivin和XIAP mRNA的表达，进而抑制SMMC-7721细胞的增殖，诱导其凋亡。
Abstract:
Objective    To investigate the effect of periplocin of cortex periplocae (CPP) on Stat3 signaling and its probable molecular mechanism of inducing apoptosis and anti-tumor activity.     Methods    Cell proliferation was detected by MTT. Cell apoptosis and cell cycle were investigated by flow cytometry. Expression of Stat3 protein in SMMC-7721 cells was analyzed by Western blot. Mcl-1, Survivin and XIAP mRNA expressions were measured by RT-PCR.     Results    CPP inhibited the proliferation of SMMC-7721 cells significantly, induced their apoptosis and arrested their cell cycle at G2/M phase. Decreased expression of Stat3 protein in the cell nucleus was observed after CPP treatment, but no significant changes were found in cytoplasma. Mcl-1, Survivin and XIAP mRNA expression levels were decreased in a time-dependent manner.     Conclusion    CPP inhibits cell proliferation and induces apoptosis by inhibiting Stat3 signal transduction in human hepatocellular carcinoma cell line SMMC-7721.

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更新日期/Last Update: 2008-07-28