[1]路扬,李龙坤,王永权.牵张负荷对大鼠膀胱ICCs细胞数量及c-kit表达的影响[J].第三军医大学学报,2008,30(07):570-573.
 LU Yang,LI Long-kun,WANG Yong-quan.Effects of stretch load on quantity and c-kit expression of rat bladder interstitial cells of Cajal[J].J Third Mil Med Univ,2008,30(07):570-573.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
30卷
期数:
2008年第07期
页码:
570-573
栏目:
专题报道
出版日期:
2008-04-15

文章信息/Info

Title:
Effects of stretch load on quantity and c-kit expression of rat bladder interstitial cells of Cajal
作者:
路扬李龙坤王永权
第三军医大学西南医院全军泌尿外科研究所
Author(s):
LU Yang LI Long-kun WANG Yong-quan
Urinary Surgery Research Institute, Southwest Hospital, Third Military Medical University
关键词:
牵张负荷 Cajal间质细胞 c-kit膀胱
Keywords:
stretch load interstitial cells of Cajal c-kit bladder
分类号:
R322.62; R329.25; R394.2
文献标志码:
A
摘要:
目的 探讨牵张负荷条件对膀胱Cajal间质细胞(interstitial cells of Cajal, ICCs)数量及c-kit蛋白表达变化的影响,阐明牵张负荷对膀胱ICCs细胞兴奋性的调控作用。 方法 构建大鼠不稳定逼尿肌模型,以光镜计数及免疫组化法检测牵张负荷下逼尿肌稳定组和逼尿肌不稳定组与正常对照组膀胱ICCs细胞数量及酪氨酸激酶受体(c-kit)在蛋白水平表达的差异。 结果 逼尿肌稳定组和逼尿肌不稳定组ICCs细胞数量与c-kit表达量较正常对照组均有增高(P<001),逼尿肌不稳定组较稳定组升高显著(P<0.01)。 结论 牵张负荷对ICCs细胞有调控作用; ICCs细胞数量及c-kit表达增多可能是其机制之一。
Abstract:
Objective To investigate the effects of stretch load on the quantity of rat bladder interstitial cells of Cajal (ICCs) and ckit expression in the cells.  Methods Rat models of detrusor instability with partial outflow obstruction were established. The quantity of ICCs and c-kit expression in ICCs from stable, unstable and normal detrusor were studied with light microscopy and Western blotting.  Results The quantity of ICCs and expression of c-kit in the stable and unstable detrusors were higher than that in normal control group (P<0.01), and those of the unstable detrusor were significant higher than those of the stable one (P<0.01).  Conclusion Stretch load can regulate the function of ICCs, probably through increment of the quantity of ICCs and up-regulation of c-kit expression in the cells.

参考文献/References:

路扬,李龙坤,王永权. 牵张负荷对大鼠膀胱ICCs细胞数量及c-kit表达的影响[J]. 第三军医大学学报,2008,30(7):570-573.

相似文献/References:

[1]王永权,方强,路扬,等.牵张负荷对豚鼠膀胱ICCs细胞内钙波的影响[J].第三军医大学学报,2008,30(07):574.
 WANG Yong-quan,FANG Qiang,LU-Yang,et al.Effect of stretch load on calcium wave of interstitial cells of Cajal in guinea pig bladder[J].J Third Mil Med Univ,2008,30(07):574.
[2]路扬,李龙坤,王永权.牵张负荷对大鼠膀胱Cajal间质细胞超微形态及c-kit基因表达影响[J].第三军医大学学报,2009,31(13):1284.
 LU Yang,LI Long-kun,WANG Yong-quan.Effects of stretch load on the ultramicrostructure and c-kit gene expression of the bladder ICCs in rats[J].J Third Mil Med Univ,2009,31(07):1284.

更新日期/Last Update: 2008-04-16