[1]沈文浩,熊恩庆,宋波,等.成年豚鼠膀胱ICCs细胞起搏电流的鉴定[J].第三军医大学学报,2008,30(07):567-569.
 SHEN Wen-hao,XIONG En-qing,SONG Bo,et al.Identification of pacemaker-like current in interstitial cells of Cajal in bladder of adult guinea pigs[J].J Third Mil Med Univ,2008,30(07):567-569.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
30卷
期数:
2008年第07期
页码:
567-569
栏目:
专题报道
出版日期:
2008-04-15

文章信息/Info

Title:
Identification of pacemaker-like current in interstitial cells of Cajal in bladder of adult guinea pigs
作者:
沈文浩熊恩庆宋波李为兵卢根生李龙坤金锡御
第三军医大学西南医院全军泌尿外科研究所
Author(s):
SHEN Wen-hao XIONG En-qing SONG Bo LI Wei-bing LU Gen-sheng LI Long-kun JIN Xi-yu
Urinary Surgery Research Institute, Southwest Hospital, Third Military Medical University
关键词:
Cajal间质细胞起搏电流膀胱
Keywords:
interstitial cells of Cajal pacemaker current bladder
分类号:
R322.62; R329.27; R337.5
文献标志码:
A
摘要:
目的 确定成年豚鼠膀胱卡哈尔间质细胞(interstitial cells of Cajal, ICCs)细胞是否具有起搏电流。方法 采用胶原酶消化法,得到原代培养的成年豚鼠膀胱ICCs细胞。采用膜片钳技术,在全细胞记录模式下,记录其超级化激活的内向电流(hyperrepolarization-activated inward current, Ih)。结果 原代培养的ICCs细胞和逼尿肌细胞的静息膜电位、膜电容存在明显差异;ICCs细胞可记录到起搏细胞特征电流Ih,而在逼尿肌细胞则记录不到。 结论 记录到成年豚鼠膀胱ICCs细胞具有起搏细胞的特征电流Ih,提示ICCs细胞在膀胱具有起搏作用。
Abstract:
Objective To ascertain the presence of pacemaker-like current in interstitial cells of Cajal (ICCs) in the bladder of adult guinea pigs. Methods Primarily cultured bladder ICC of adult guinea pigs were harvested by collagenase digestion. Hyperrepolarization-activated inward current (Ih) and pacemaker specific current were recorded using whole-cell patch clamp technique. Results There was significant difference in resting membrane potential and membrane capacitance between ICCs and detrusor myocytes. Ih was confirmed in the ICCs, but not in the detrusor myocytes. Conclusion We have confirmed the existence of the specific pacemaker current in the ICCs in the bladder of adult guinea pigs, implying that ICCs might play an important role as pacemaker in the bladder.

参考文献/References:

沈文浩,熊恩庆,宋波,等. 成年豚鼠膀胱ICCs细胞起搏电流的鉴定[J]. 第三军医大学学报,2008,30(7):567-569.

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更新日期/Last Update: 2008-04-16