[1]邓莉,张轩珍,王卫东,等.重组人内皮抑素联合紫杉醇+顺铂化疗方案治疗人乳腺癌祼鼠移植瘤的研究[J].第三军医大学学报,2008,30(09):783-786.
 DENG Li,ZHANG Xuan-zhen,WANG Wei-dong,et al.Recombinant human endostatin combined with paclitaxel-cisplatin chemotherapy in treatment of human breast cancer xenograft in nude mice[J].J Third Mil Med Univ,2008,30(09):783-786.
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重组人内皮抑素联合紫杉醇+顺铂化疗方案治疗人乳腺癌祼鼠移植瘤的研究(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
30卷
期数:
2008年第09期
页码:
783-786
栏目:
论著
出版日期:
2008-05-15

文章信息/Info

Title:
Recombinant human endostatin combined with paclitaxel-cisplatin chemotherapy in treatment of human breast cancer xenograft in nude mice
作者:
邓莉张轩珍王卫东陈正堂曹正怀
第三军医大学新桥医院全军肿瘤诊治研究所;重庆市妇幼保健院病理科
Author(s):
DENG Li ZHANG Xuan-zhen WANG Wei-dong CHEN Zheng-tang CAO Zheng-huai
Cancer Research Center, Xinqiao Hospital, Third Military Medical University; Department of Pathology, Women’s and Children’s Health Care Hospital of Chongqing
关键词:
乳腺癌重组人内皮抑素化疗裸鼠
Keywords:
breast cancerrecombinant human endostatinchemotherapynude mice
分类号:
R73-36; R737.9; R979.1
文献标志码:
A
摘要:
目的  探讨重组人内皮抑素(endostar,商品名恩度)联合化疗方案对人乳腺癌(MCF-7)裸鼠模型的治疗,观察其对祼鼠血管生成及肿瘤生长的抑制作用。  方法  将48只荷瘤鼠按随机数字表法分为A组及B组,每组24只,两组再分为化疗组、内皮抑素组、联合用药组及对照组,化疗组给予紫杉醇+顺铂(TP),紫杉醇20 mg/kg,腹腔注射,第1、7、14天,顺铂5 mg/kg,腹腔注射,第1、7天;内皮抑素组给予重组人内皮抑素10 mg/kg,静脉推注,连续14 d;联合用药组给予重组人内皮抑素+TP:紫杉醇20 mg/kg,腹腔注射,第1、7、14天,顺铂5 mg/kg,腹腔注射,第1、7天,重组人内皮抑素10 mg/kg,静脉推注;对照组连续14 d静脉推注等体积生理盐水,比较各组的抑瘤率,绘制肿瘤生长曲线。ELISA方法检测血清血管内皮生长因子(VEGF),肿瘤组织病理切片,观察微血管密度(MVD)、凋亡指数,B组动物观察其生存期。  结果  联合用药组VEGF、MVD与其他各组比较明显减低(P<0.05),凋亡指数与其他各组比较明显增加(P<0.05),生存时间与对照组比较明显延长(P<0.05)。  结论  重组人内皮抑素联合TP方案治疗人乳腺癌裸鼠移植瘤对肿瘤生长及肿瘤血管抑制优于单一化疗,但1个周期联合化疗用药不能延长生存期。
Abstract:
Objective  To investigate the anti-angiogenesis and tumor inhibitory effects of recombinant human endostatin (rhES) combined with paclitaxel-cisplatin chemotherapy (TP regimen) on xenograft tumors in nude mice of human breast carcinoma cell MCF-7.   Methods  Forty-eight xenograft nude mice were randomized into group A and group B,then every group were divided 4 subgroups, that is, TP group (cisplatin 20 mg/kg at days 1, 7 and 14, and paclitaxel 5 mg/kg at days 1 and 7, i.p.), rhES group (rhES 10 mg·kg-1·d-1 for 14 d, i.v.), rhES+TP group (combined treatment as abovementioned), and control group (normal saline, i.v.). Inhibitory rate of xenograft and tumor growth curve was calculated and plotted. Serum VEGF level was determined by ELISA, microvessel density (MVD) in tumors was measured by immunohistochemistry, and cell apoptosis was examined by TUNEL staining. Survival time was observed in group B.   Results  Serum VEGF and MVD was significantly lower in rhES+TP group than in other groups (P<0.05), but its apoptosis index was higher (P<0.05). Survival time had no difference in rhES+TP group than in TP group, but was longer than that of control group (P<0.05).   Conclusion  rhES combined TP regimen is better than TP regimen in tumor growth and microvessel growth of human breast cancer xenograft, but the survival is not significant prolonged within one treatment cycle.

参考文献/References:

邓莉,张轩珍,王卫东,等. 重组人内皮抑素联合紫杉醇+顺铂化疗方案治疗人乳腺癌祼鼠移植瘤的研究[J]. 第三军医大学学报,2008,30(9):783-786.

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更新日期/Last Update: 2008-05-07