[1]李招权,司维柯,邹全明,等.外源性Wnt5a激活K562细胞非经典Wnt5a/Ca2+信号途径研究[J].第三军医大学学报,2008,30(10):924-927.
　LI Zhao-quan,SI Wei-ke,ZOU Quan-ming,et al.Noncanonical Wnt5a/Ca2+ pathway activated by exogenous Wnt5a in K562 cells[J].J Third Mil Med Univ,2008,30(10):924-927.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
30卷
期数:
2008年第10期
页码:
924-927
栏目:
论著
出版日期:
2008-05-30

文章信息/Info

Title:
Noncanonical Wnt5a/Ca2+ pathway activated by exogenous Wnt5a in K562 cells
作者:
李招权; 司维柯; 邹全明; 袁媛; 潘静; 赵宸
第三军医大学医学检验系：临床血液学教研室，临床微生物学及免疫学教研室，重庆市生物制药工程技术研究中心
Author(s):
LI Zhao-quan;  SI Wei-ke;  ZOU Quan-ming;  YUAN Yuan;  PAN Jing;  ZHAO Chen
Department of Clinical Hematology, Department of Clinical Microbiology and Immunology, College of Medical Laboratory, Third Military Medical University
关键词:
Wnt5a; K562细胞; Ca2+; β-catenin; cyclin D1
Keywords:
Wnt5a; K562 cells; Ca2+; β-catenin; cyclin D1
分类号:
R329.28;R730.33;R733.7
文献标志码:
A
摘要:
目的  观察外源性Wat5a对K562细胞非经典Wnt5a/Ca2+途径的影响，进一步研究外源性Wnt5a诱导K562细胞的分化机制。  方法  激光共聚焦显微镜观察Ca2+内流的变化，以RT-PCR及免疫组化检测β-catenin的表达变化，以Western blot检测cyclin D1的表达变化。  结果  外源性Wnt5a能明显促进K562细胞Ca2+内流，显著下调cyclin D1表达，且能下调K562细胞β-catenin表达，但β-catenin表达下调无统计学意义。  结论  外源性Wnt5a能激活K562细胞非经典Wnt5a/Ca2+途径，外源性Wnt5a诱导K562细胞分化机制可能与此相关。
Abstract:
Objective  To study the mechanism of K562 cell differentiation induced by exogenous Wnt5a in aspect of noncanonical Wnt5a/Ca2+ signaling pathway.   Methods  After K562 cells were cultured in medium supplemented with Wnt5a- or GFP-riched supernatant for 1-5 d, Ca2+ inflow changes were observed by laser confocal microscopy. The expression changes of β-catenin and cyclin D1 were detected by RT-PCR, immunocytochemical staining and Western blotting.   Results  Exogenous Wnt5a obviously promoted the Ca2+ inflow of K562 cells and down-regulated the expression of cyclin D1. Furthermore, the expression of β-catenin was lower, but had no statistically significance.   Conclusion  Exogenous Wnt5a can activate noncanonical Wnt5a/Ca2+ signaling pathway in K562 cells, which may relate to the K562 cell differentiation.

参考文献/References:

李招权，司维柯，邹全明，等. 外源性Wnt5a激活K562细胞非经典Wnt5a/Ca2+信号途径研究[J]. 第三军医大学学报，2008，30（10）：924-927.

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更新日期/Last Update: 2008-05-26