[1]陈琳,徐酉华,温贤浩,等.RNA干扰survivin基因对K562细胞化疗敏感性的影响及机制[J].第三军医大学学报,2008,30(22):2111-2114.
 CHEN Lin,XU You-hua,WEN Xian-hao,et al.Effect of RNA-silenced survivin gene on sensitivity of K562 cells to adriamycin[J].J Third Mil Med Univ,2008,30(22):2111-2114.
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RNA干扰survivin基因对K562细胞化疗敏感性的影响及机制(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
30卷
期数:
2008年第22期
页码:
2111-2114
栏目:
论著
出版日期:
2008-11-30

文章信息/Info

Title:
Effect of RNA-silenced survivin gene on sensitivity of K562 cells to adriamycin
作者:
陈琳徐酉华温贤浩杨山于洁戴碧涛李欣
重庆医科大学儿童医院:血液科,临床检验中心
Author(s):
CHEN Lin XU You-hua WEN Xian-hao YANG Shan YU Jie DAI Bi-tao LI Xin
Department of Hematology, Center of Clinical Laboratory, Children’s Hospital, Chongqing Medical University, Chongqing 400014, China
关键词:
K562细胞RNA干扰survivin阿霉素耐药蛋白
Keywords:
K562 cells RNA interference survivin ADM drug resistance-protein
分类号:
R394.3;R73-361;R733.7
文献标志码:
A
摘要:
目的    应用小分子干扰RNA(siRNA)技术抑制survivin基因的表达,观察其对K562细胞化疗敏感性的影响,为临床白血病治疗提供理论依据。    方法    实验分为RNA干扰组、脂质体对照组、空白对照组。转染48 h后用RT-PCR法,免疫组化法分别检测survivin在mRNA及蛋白水平的表达;MTT法检测细胞对阿霉素的敏感性;流式细胞仪检测细胞内阿霉素的药物浓度;免疫组化法检测K562细胞中3种耐药相关蛋白P-gp、TOPO-2、GST-π的表达。    结果    ①RNA干扰组K562细胞的survivin基因表达水平明显下降;②RNA干扰组K562细胞对阿霉素敏感性增高;③RNA干扰组K562细胞中阿霉素浓度增高;④RNA干扰组K562细胞耐药蛋白GST-π表达水平下降, 而P-gp,TOPO-2的表达与对照组比较无显著差异。    结论    特异性siRNA能够有效抑制survivin基因的表达,并可能通过降低耐药蛋白GST-π的表达增加K562细胞对阿霉素的敏感性。
Abstract:
Objective    To explore the effects of survivin-targeted small interference RNA (siRNA) on the sensitivity of K562 cell line to adriamycin (ADM).     Methods    K562 cells were divided into three groups: RNA interference (RNAi) group, lipofectamine group and control group. Cell transfection was induced by lipofectamine2000. The expressions of survivin in K562 cells were assayed 48 h after transfection by RT-PCR and immunohistochemical methods. The protein levels of P-glycoprotein (P-gp), TOPO-2, GST-π were detected using immunohistochemical methods. The sensitivity of K562 cells to ADM was determined by MTT and flow cytometry.     Results    After transfection with recombinant plasmid pshRNA-survivin, the mRNA and protein expressions of survivin in K562 cells were decreased significantly (P<0.05). The sensitivity of K562 cells to ADM was increased in RNAi group as compared with lipofectamine group and control group (P<0.05). The protein level of GST-π expression was significantly lower in RNAi group than those in the other two control groups (P<0.05).     Conclusion    Survivin-targeted RNA interference can increase the sensitivity of K562 cells to ADM, probably by decreasing the expression of GST-π.

参考文献/References:

陈琳, 徐酉华, 温贤浩, 等. RNA干扰survivin基因对K562细胞化疗敏感性的影响及机制[J]. 第三军医大学学报,2008,30(22):2111-2114.

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更新日期/Last Update: 2008-10-30