[1]邓永红,李军梅,刘刚.低频复合生理频率慢性电刺激对肺气肿兔膈肌力学特性的影响[J].陆军军医大学学报(原第三军医大学学报),2008,30(06):510-513.
 DENG Yong-hong,LI Jun-mei,LIU Gang.Effect of chronic electrical stimulus of low and physiological frequency on diaphragmatic mechanical characteristics in rabbits with emphysema[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(06):510-513.
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低频复合生理频率慢性电刺激对肺气肿兔膈肌力学特性的影响(/HTML )
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陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
30卷
期数:
2008年第06期
页码:
510-513
栏目:
论著
出版日期:
2008-03-30

文章信息/Info

Title:
Effect of chronic electrical stimulus of low and physiological frequency on diaphragmatic mechanical characteristics in rabbits with emphysema
文章编号:
1000-5404(2008)06-0510-04
作者:
邓永红李军梅刘刚
第三军医大学西南医院呼吸内科
Author(s):
DENG Yong-hong LI Jun-mei LIU Gang
Department of Respiratory Diseases, Southwest Hospital, Third Military Medical University
关键词:
肺气肿膈肌慢性电刺激MHC亚型力学
Keywords:
emphysema diaphragm muscle chronic electrical stimulation subtype of myosin heavy chain mechanics
分类号:
R332.1;R454.1;R563.3
文献标志码:
A
摘要:
目的 研究低频复合生理频率慢性电刺激后肺气肿兔膈肌肌球蛋白重链(myosin heavy chain, MHC)亚型及其力学模式的适应性变化。 方法 采用木瓜蛋白酶雾化吸入法建立肺气肿模型:测定正常对照组、肺气肿组和肺气肿各慢性电刺激(chronic electrical stimulation, CES)组膈肌MHC亚型及膈肌颤搐收缩张力(Pt)、峰值张力时间(TPT)、1/2松弛时间(1/2Rt)、强直颤搐收缩张力(Po)、疲劳指数(FI)和疲劳恢复指数(FRI)的适应性变化。 结果 ①40 Hz、10 Hz、(10+40) Hz慢性电刺激后肺气肿兔膈肌MHC的相对含量较刺激前都明显增加(P<0.05)。10 Hz组MHC-I型比例明显增加(P<0.01),(10+40) Hz组MHC-I、MHC-IIa型比例增加(P<0.05)。②肺气肿组Pt、Po均较正常对照组明显减低(P<0.01),TPT、1/2Rt延长(P<0.01),FI、FRI增加(P<0.01);(10+40) Hz组和40 Hz组与肺气肿组比较Pt、Po均明显增加(P<0.01),TPT、1/2Rt均明显缩短(P<0.01),FI和FRI下降(P<0.01);(10+40) Hz组与40 Hz组比较,Pt、Po、TPT、1/2Rt无显著性差异(P>0.05),FI和FRI降低(P<0.01)。 结论 不同频率的CES可导致膈肌MHC亚型及肌条力学产生不同的适应性变化,低频复合生理频率电刺激可显著提高肺气肿兔膈肌收缩力,增强膈肌抗疲劳能力,可能是体外膈肌起搏对COPD患者膈肌康复治疗更佳的频率模式之一。
Abstract:
Objective To investigate the effect of chronic electrical stimulus (CES) of low frequency and physiological frequency on diaphragmatic subtype of myosin heavy chain (MHC) and mechanical characteristic in rabbits with emphysema.  Methods The rabbit model of emphysema was made by inhaling papain of ultrasonic atomization once a week for 3 weeks in 30 adult Japanese rabbits, 6 receiving 10 Hz chronic low-frequency electrical stimulation, 6 receiving 40 Hz, 6 receiving 10 Hz plus 40 Hz. Another 6 emphysema rabbits and 6 normal rabbits served as control. The twitch tension (Pt), titanic tension (Po), time to peak tension (TPT), half-relaxation time (1/2Rt), fatigue index (FI) and fatigue recovery index (FRI) were measured and SDSpolyacrylamide gel electrophoresis was used to observe the changes in subtype of MHC in control group, emphysema group, CES groups respectively.  Results ①Following chronic electrical stimulus of 40 Hz, 10 Hz, and (10+40) Hz, the relative contents of MHC were increased in diaphragmatic muscle of rabbits with emphysema. The percentage of MHC-I was significantly increased in the 10 Hz group, while that of MHC-I and MHC-IIa were significantly increased in the (10+40) Hz group. ②Following chronic electrical stimulation using 40 Hz and (10+40)Hz, diaphragmatic muscle Pt and Po were significantly increased in rabbits with emphysema, TPT and 1/2Rt were significantly reduced, and FI and FRI were significantly decreased (P<0.01). In the 10 Hz group, Pt and Po were reduced (P<0.05), TPT and 1/2Rt increased, and FI and FRI decreased (P<0.01). FRI and the decrease of FRI were more significant in the (10+40)Hz group than in the 40 Hz group (P<0.01).  Conclusion Different frequency electrical stimulation induced different adaptative changes in MHC and mechanical characteristic. Chronic electrical stimulation using low and physiological frequencies can increase diaphragmatic muscle inotropy and the fatigue tolerance of diaphragmatic muscle. It indicates that it may be the optimal frequency mode for the rehabilitation care of diaphragmatic muscle of COPD patients with extraorgan diaphragm pacing.

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更新日期/Last Update: 2008-04-07