[1]赵洪雯,余荣杰,彭侃夫,等.VEGF165基因转染EPCs移植对大鼠阿霉素肾病组织学的影响[J].第三军医大学学报,2008,30(02):93-97.
 ZHAO Hong-wen,YU Rong-jie,PENG Kan-fu,et al.Amelioration of Adriamycin nephropathy by transplanting endothelial progenitor cells transfected with Ad-VEGF165 gene[J].J Third Mil Med Univ,2008,30(02):93-97.
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VEGF165基因转染EPCs移植对大鼠阿霉素肾病组织学的影响(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
30卷
期数:
2008年第02期
页码:
93-97
栏目:
专题报道
出版日期:
2008-01-30

文章信息/Info

Title:
Amelioration of Adriamycin nephropathy by transplanting endothelial progenitor cells transfected with Ad-VEGF165 gene
作者:
赵洪雯余荣杰彭侃夫刘宏吴雄飞贺伟峰张晓蓉
第三军医大学西南医院:肾科,全军烧伤研究所,创伤、烧伤与复合伤国家重点实验室,疾病蛋白质组学重庆市市级重点实验室
Author(s):
ZHAO Hong-wen YU Rong-jie PENG Kan-fu LIU Hong WU Xiong-fei HE Wei-feng ZHANG Xiao-rong
Department of Kidney, State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Burns, Chongqing Key Laboratory for Proteomics of Diseases, Southwest Hospital, Third Military Medical University
关键词:
血管内皮生长因子腺病毒内皮祖细胞阿霉素肾病
Keywords:
vascular endothelial growth factor adenovirus endothelial progenitor cells Adriamycin nephropathy
分类号:
R329;R394.2;R692.02
文献标志码:
A
摘要:
目的 构建含血管内皮生长因子165基因的重组腺病毒(Ad-VEGF165),转染血管内皮祖细胞(EPCs)后,观察其对阿霉素肾病大鼠肾脏组织学的影响。  方法 用细菌内同源重组法构建含VEGF165基因的重组腺病毒Ad-VEGF165,通过PCR和Western blot鉴定所构建的腺病毒。贴壁法体外培养获得大鼠骨髓来源的EPCs,Ad-VEGF165体外转染EPCs,检测转染后目的基因的蛋白表达。大鼠阿霉素肾病模型形成过程中,尾静脉注射转染Ad-VEGF165的EPCs,在第16周时观察肾脏组织学变化。  结果 PCR和Western blot证实构建的重组腺病毒含有目的基因,滴度为1.4×1010 PFU/ml;培养第6天的EPCs用于Ad-VEGF165转染,转染后24 h,培养上清中VEGF蛋白表达较对照组和转染前增加。转染了VEGF165基因的EPCs移植后第12周(切肾术后16周),转染组的肾小球硬化和肾小管间质纤维化的程度明显较肾病组轻。  结论 转染了VEGF165基因的EPCs可以减轻阿霉素肾病的肾小球和肾小管的损伤程度,为VEGF在慢性肾脏病治疗提供了实验基础。
Abstract:
Objective To construct the recombinant adenovirus of vascular endothelial growth factor 165 (Ad-VEGF165) and observe the effects of endothelial progenitor cells (EPCs) transfected with Ad-VEGF165 gene on ameliorating renal damages induced with Adriamycin.   Methods Ad-VEGF165  was identified with polymerase chain reaction (PCR) and Western blot. EPCs derived from rat bone marrow were obtained by adherent culture for 6 days and transfected with Ad-VEGF165, then injected into tail vein at the early stage of Adriamycin induced renal disease.   Results PCR and Western blot indicated that the recombinant adenovirus contained the inserted VEGF165. The titer of the purified Ad-VEGF165  was 1.4×1010 pfu/ml. After transfection, the expression of VEGF protein was significantly higher than that of control and untransfected EPCs. The pathological changes were less in VEGF165 transfecting group than that in untransfecting group at the 12th week, including glomerular sclerosis and tubulointerstitial fibrosis induced by Adriamycin after transplantation.   Conclusion The Ad-VEGF165 effectively mediates the target gene expression in cultured EPCs and ameliorates renal damages. But the adverse effect of VEGF to renal function needs further study.

参考文献/References:

赵洪雯,余荣杰,彭侃夫,等. VEG165基因转染EPCs移植对大鼠阿霉素肾病组织学的影响[J].第三军医大学学报,2008,30(2):93-97.

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更新日期/Last Update: 2008-04-29