[1]刘东宁,阴正勤,吴楠,等.Long-Evans大鼠骨髓间充质干细胞分离培养及生物学特性研究[J].陆军军医大学学报(原第三军医大学学报),2008,30(15):1441-1443.
 LIU Dong-ning,YIN Zheng-qin,WU Nan,et al.Isolation, cultivation and identification of Long-Evans rat bone mesenchymal stem cells[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(15):1441-1443.
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Long-Evans大鼠骨髓间充质干细胞分离培养及生物学特性研究(/HTML )
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陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
30卷
期数:
2008年第15期
页码:
1441-1443
栏目:
论著
出版日期:
2008-08-15

文章信息/Info

Title:
Isolation, cultivation and identification of Long-Evans rat bone mesenchymal stem cells
作者:
刘东宁阴正勤吴楠王艳华
第三军医大学西南医院全军眼科中心;沈阳市第四人民医院眼科
Author(s):
LIU Dong-ning YIN Zheng-qin WU Nan WANG Yan-hua
Department of Ophthalmology, Southwest Hospital, Third Military Medical University, Chongqing 400038; The Fourth People’s Hospital of Shenyang, Shenyang 110031, China
关键词:
骨髓间充质干细胞细胞培养生物学特性
Keywords:
bone mesenchymal stem cells cell culture biological characteristics
分类号:
R329-33; R329.25; R331.22
文献标志码:
A
摘要:
目的  对Long-Evans大鼠骨髓间充质干细胞(bone mesenchymal stem cells, BMSCs)进行分离培养,并检测其生物学特性。 方法  采用0.85%NH4Cl分离培养Long-Evans大鼠股骨来源BMSCs,检测生长曲线和生长周期,并进行细胞表面抗原和成骨能力鉴定。 结果  BMSCs体外培养生长状况良好,具有活跃的增殖能力。第5代细胞生长速度较第3代略减慢。细胞周期显示93.79%细胞处于G0~G1期。表面抗原检测绝大多数细胞阳性表达CD29和CD90,几乎不表达CD45。在体外能够向成骨细胞分化。 结论  Long-Evans大鼠股骨来源骨髓间充质干细胞经0.85%NH4Cl分离培养后,纯度高,具有良好生物学特性。
Abstract:
Objective    To isolate and cultivate Long-Evans rat bone mesenchymal stem cells (BMSCs) and identify their biological characteristics.     Methods    BMSCs were obtained from femurs of Long-Evan rats and dealt with 0.85% NH4Cl. The cell growth curve and cell cycle were measured and surface antigens were detected by flow cytometry. Osteoblast differentiation was studied by Alizarin red staining.     Results    BMSCs had active proliferative ability. The growth velocity of passage 5 cells was slower than that of passage 3 cells. The cell cycle analysis showed that 93.79% of BMSCs was at G0/G1phase. BMSCs were positive for CD29 and CD44, but negative for CD45. Alizarin red staining of BMSCs after osteoblast induction was positive.     Conclusion    Highly purified BMSCs obtained from Long-Evan rats by 0.85% NH4Cl show stable biological properties.

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更新日期/Last Update: 2008-07-24