[1]李福兵,赵玲,鲁秀敏,等.成熟成骨细胞中敲除基因fgfr1小鼠的制备[J].第三军医大学学报,2008,30(04):280-283.
 LI Fu-bing,ZHAO Ling,LU Xiu-min,et al.Generation of differentiated osteoblast specific fgfr1 knockout mice[J].J Third Mil Med Univ,2008,30(04):280-283.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
30卷
期数:
2008年第04期
页码:
280-283
栏目:
论著
出版日期:
2008-02-28

文章信息/Info

Title:
Generation of differentiated osteoblast specific fgfr1 knockout mice
作者:
李福兵赵玲鲁秀敏余瑛何启芬陈锚锚段亚琪戚华兵陈林
第三军医大学大坪医院野战外科研究所全军创伤中心,创伤、烧伤与复合伤国家重点实验室
Author(s):
LI Fu-bing ZHAO Ling LU Xiu-min YU Ying HE Qi-fen CHEN Mao-mao DUAN Ya-qi QI Hua-bing CHEN Lin
State Key Laboratory of Trauma, Burns and Combined Injury, Trauma Center, Institute of Surgery Research, Daping Hospital, Third Military Medical University
关键词:
fgfr1条件性基因敲除小鼠骨骼发育
Keywords:
fibroblast growth factor receptor 1 conditional gene knockout mice bone development
分类号:
R-332;R322.71;R394.2
文献标志码:
A
摘要:
目的 制备在成熟成骨细胞中敲除基因fgfr1的特定细胞阶段性基因敲除小鼠。  方法 从美国NIH引进SPF级fgfr1条件性基因敲除(fgfr1flox/flox)小鼠,进行饲养、繁殖和PCR鉴定;fgfr1flox/flox小鼠与在成熟成骨细胞表达Cre重组酶的OC-Cre小鼠交配,获得在成熟成骨细胞中敲除一个基因拷贝的杂合子小鼠;杂合子小鼠之间交配,或者杂合子小鼠与fgfr1flox/flox小鼠交配,可以获得在成熟成骨细胞中敲除fgfr1基因的小鼠fgfr1△/△/OC-CreTG/+。  结果 成功制备在成熟成骨细胞中敲除fgfr1的细胞特异性敲除小鼠。  结论 成功引进fgfr1flox/flox小鼠,应用合理的基因条件性敲除繁殖策略,获得在成熟成骨细胞中敲除fgfr1的基因敲除小鼠。
Abstract:
Objective To obtain differentiated osteoblast-specific inactivation of fgfr1 mice.  Methods To obtain fgfr1△/△/OC-CreTG/+ mice, fgfr1flox/flox mice obtained from NIH were crossed with OC-Cre mice. To obtain fgfr1△/△/OC-CreTG/+ mutant mice, fgfr1△/△/OC-CreTG/+ further crossed with themselves or fgfr1flox/flox mice. After fgfr1△/△/OC-CreTG/+ crossed with fgfr1flox/flox mice, half of their offspring were mutant mice.  Results Differentiated osteoblast-specific fgfr1 knockout mice were obtained.  Conclusion  fgfr1△/△/OC-CreTG/+ mice were obtained through proper crossing strategy, which provides a suitable platform for studying fgfr1 function in bone development and fracture healing.

参考文献/References:

李福兵, 赵玲, 鲁秀敏,等. 成熟成骨细胞中敲除基因fgfr1小鼠的制备[J].第三军医大学学报,2008,30(4):280-283.

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更新日期/Last Update: 2008-04-29