[1]赵士彭,赵发,许明月,等.塞来昔布对人结肠癌裸鼠移植瘤的抑制作用及其机制[J].第三军医大学学报,2008,30(14):1338-1341.
 ZHAO Shi-peng,ZHAO Fa,XU Ming-yue,et al.Celecoxib suppresses xenograft tumor of colon cancer in nude mice[J].J Third Mil Med Univ,2008,30(14):1338-1341.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
30卷
期数:
2008年第14期
页码:
1338-1341
栏目:
论著
出版日期:
2008-07-30

文章信息/Info

Title:
Celecoxib suppresses  xenograft tumor of colon cancer in nude mice
作者:
赵士彭赵发许明月张啸尘蔡建辉
河北医科大学: 第三医院肛肠外科,河北省大肠肛门病研究中心,第二医院胃肠外科
Author(s):
ZHAO Shi-peng  ZHAO Fa XU Ming-yue ZHANG Xiao-chen CAI Jian-hui
Department of Anorectal Surgery, The Third Hospital, Shijiazhuang 050051;Department of Gastroenterology, The Second Hospital, Hebei Medical University, Shijiazhuang 050000, China
关键词:
结肠癌微血管生成环氧化酶2抑制剂survivin
Keywords:
colon cancer angiogenesis COX-2 inhibitor survivin
分类号:
R73-361;R735.35;R979.11
文献标志码:
A
摘要:
目的  研究不同剂量环氧化酶-2(COX-2)抑制剂塞来昔布对人结肠癌裸鼠移植瘤生长的影响及机制。 方法  将人结肠癌HT-29细胞注射于裸鼠背部皮下建立移植瘤模型,16 d后,将裸鼠以随机数字表法分为5组:对照组、塞来昔布1组(25 mg·kg-1·d-1)、塞来昔布2组(50 mg·kg-1·d-1)、塞来昔布3组(75 mg·kg-1·d-1)、塞来昔布4组(100 mg·kg-1·d-1),并给予相应剂量的塞来昔布。每周测量肿瘤体积,给药35 d后,处死裸鼠,切取移植瘤组织检测COX-2,survivin表达,VEGF mRNA表达和微血管密度(MVD)。 结果  塞来昔布1、2、3、4组的抑瘤率分别为34.94%、39.20%、53.50%、59.20%,与对照组比较移植瘤体积明显缩小(P<0.05)。与对照组比较,塞来昔布各组COX-2、survivin的表达水平均明显降低(P<0.05),并且抑制程度呈药物剂量依赖性。低剂量(25 mg·kg-1·d-1)塞来昔布可明显抑制VEGF mRNA表达和结肠癌移植瘤微血管生成(P<0.05, vs 对照组),其抑制作用并未随用药剂量的增加而增强。 结论  不同剂量的塞来昔布均能明显抑制结肠癌移植瘤的生长,其抑制作用呈用药剂量依赖性,这种作用可能是通过抑制survivin的表达实现的。低剂量塞来昔布可抑制人结肠癌裸鼠移植瘤微血管生成,可能对防止肿瘤转移起到重要作用。
Abstract:
Objective    To evaluate the effects of celecoxib on tumor growth,COX-2 and survivin expressions and angiogenesis in nude mice.     Methods    Xenograft animal model was established by injecting human colon cancer HT-29 cells into the BALB/c nude mice subcutaneously.  Fifty mice were randomly divided into 4 groups 16 d after injection:control group, celecoxib group(receiving 25, 50, 75, 100 mg·kg-1·d-1 for 35 d). Tumor volumes were measured every week.  The expression level of COX-2, survivin and the microvessel density (MVD) of the xenograft tumor tissues were measured by immunohistochemistry, and mRNA level of VEGF by RT-PCR.     Results    Celecoxib at dose of 25, 50, 75 and 100 mg·kg-1·d-1 inhibited the tumor volume by 34.94%, 39.20%, 53.50%, 59.20% respectively, and showed more effective in suppressing the tumor growth than the control group(P<0.05).  All celecoxib groups showed a significantly decreased expression of COX-2 and survivin compared with those in the control group (P<0.05) and their expression was in a dose-dependent manner. 25 mg·kg-1·d-1 celecoxib inhibited VEGF mRNA expression and angiogenesis as compared with the control group (P<0.05), but not in a dose-dependent manner.     Conclusion    Celecoxib can suppress tumor growth partly by inhibiting survivin expression. Low dose of celecoxib can decrease the angiogenesis of xenograft colon cancer, and furthur more prevent the metastasis of  colorectal cancer.

参考文献/References:

赵士彭,赵发,许明月,等.  塞来昔布对人结肠癌裸鼠移植瘤的抑制作用及其机制[J].第三军医大学学报,2008,30(14):1338-1341.

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更新日期/Last Update: 2008-07-21