[1]陈婷,万瑛,汤旭东,等.人肿瘤转移相关抗原肝素酶HLA-A2.1限制性CTL表位的预测及初步鉴定[J].第三军医大学学报,2007,29(22):2120-2123.
 CHEN Ting,WAN Ying,TANG Xu-dong,et al.Prediction and peptide binding assay of HLA-A2.1-restricted CTL epitope from heparanase[J].J Third Mil Med Univ,2007,29(22):2120-2123.
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人肿瘤转移相关抗原肝素酶HLA-A2.1限制性CTL表位的预测及初步鉴定(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
29卷
期数:
2007年第22期
页码:
2120-2123
栏目:
论著
出版日期:
2007-11-30

文章信息/Info

Title:
Prediction and peptide binding assay of HLA-A2.1-restricted CTL epitope from heparanase
作者:
陈婷万瑛汤旭东房殿春余松涛熊震罗元辉杨仕明
第三军医大学:西南医院全军消化病研究所,基础医学部全军免疫学研究所
Author(s):
CHEN Ting WAN Ying TANG Xu-dong FANG Dian-chun YU Song-tao XIONG Zhen LUO Yuan-hui YANG Shi-ming
Gastroenterology Research Center, Southwest Hospital, Institute of Immunology, College of Medicine, Third Military Medical University, Chongqing 400038, China
关键词:
肝素酶表位HLA-A2.1限制性分子模拟
Keywords:
heparanase epitope HLA-A2.1-restricted molecular modeling
分类号:
R394-33;R394.3;R730.2
文献标志码:
A
摘要:
目的  预测并鉴定肿瘤转移相关抗原肝素酶来源的HLA-A2.1限制性CTL表位。  方法  采用超基序、量化基序和分子模拟相结合的方法,对肝素酶HLA-A2.1限制性CTL表位进行预测,合成候选表位肽;利用T2细胞的特点,对合成的候选肽与HLA-A2.1分子进行亲和力分析,初步验证预测结果;利用标准51Cr释放试验检测特异性CTLs诱导活性。  结果  在所筛选的5个候选CTL表位中, Hpa(525-533)、Hpa(277-285)、Hpa(405-413)可在体外有效诱导肝素酶特异性CTLs的产生,对肝素酶阳性且HLA-A2阳性的KATOⅢ细胞具有明显的杀伤效应。  结论  Hpa(525-533)、Hpa(277-285)、Hpa(405-413)有可能是肿瘤抗原肝素酶HLA-A2.1的限制性CTL 表位。
Abstract:
Objective    To predict and identify the HLA-A2.1-restricted cytotoxic T lymphocyte (CTL) epitopes in heparanase (Hpa), a new tumor associated antigen.     Methods    Supermotif and quantitative motif methods were used to predict the CTL epitopes. Then the prediction was evaluated by T2 binding test. Results    Five predicted highest score candidate epitopes were Hpa(525-533), Hpa(353-361), Hpa(277-285), Hpa(400-408) and Hpa(405-413). The results of molecular simulations showed that the 5 candidate peptides could effectively bind with HLA-A2.1. Further peptide binding assay demonstrated that these 5 candidate peptides bound to HLA-A2.1 with high affinity. Hpa (525-533), Hpa(277-285) and Hpa(405- 413) induced heparanase-specific immune responses against KATO-Ⅲ gastric tumor cells, which was both positive for heparanase and HLA-A2.     Conclusion    Hpa(525-533), Hpa(277-285) and Hpa(405-413) might be the HLA-A2.1- restricted epitope of Hpa.

参考文献/References:

陈婷,万瑛,汤旭东,等.  人肿瘤转移相关抗原肝素酶HLA-A2.1限制性CTL表位的预测及初步鉴定[J]. 第三军医大学学报, 2007, 29(22):2120-2123.

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更新日期/Last Update: 2008-07-01