[1]杨松华,赵国强,郑红,等.RNAi干扰食管癌EC9706细胞MTA1基因表达对侵袭和迁移的影响[J].陆军军医大学学报(原第三军医大学学报),2008,30(06):503-505.
 YANG Song-hua,ZHAO Guo-qiang,ZHENG Hong,et al.RNAi silencing MTA1 gene inhibits invasion and migration of esophageal carcinoma 9706 cell[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(06):503-505.
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陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
30卷
期数:
2008年第06期
页码:
503-505
栏目:
论著
出版日期:
2008-03-30

文章信息/Info

Title:
RNAi silencing MTA1 gene inhibits invasion and migration of esophageal carcinoma 9706 cell
文章编号:
1000-5404(2008)06-0503-03
作者:
杨松华赵国强郑红赵继敏董子明
郑州大学基础医学院病理生理教研室
Author(s):
YANG Song-hua ZHAO Guo-qiang ZHENG Hong ZHAO Ji-min DONG Zi-ming
College of Basic Medical Science, Zhengzhou University
关键词:
MTA1RNA干扰侵袭迁移
Keywords:
MTA1 RNA interference invasion migration
分类号:
R394.3;R73-37;R735.1
文献标志码:
A
摘要:
目的 采用RNAi抑制食管癌EC9706细胞株MTA1基因的表达,观察对食管癌细胞侵袭和迁移的影响。  方法 用脂质体包裹转染沉默MTA1表达的siRNA表达载体入食管癌细胞EC9706。定量RT-PCR和免疫印迹分别检测MTA1 mRNA和蛋白表达情况;划痕损伤实验和细胞体外侵袭实验分别测定迁移和侵袭的影响。  结果 定量RT-PCR检测显示, MTA1基因的表达水平明显降低;转染沉默MTA1的siRNA表达载体组的食管癌细胞划痕未愈合, 穿透Matrigel的细胞数量明显减少,与未转染组、转染空载体组和转染无关序列siRNA载体组比较差异有显著性(P<0.05)。 结论 RNA 干扰介导的MTA1基因表达沉寂可有效降低食管癌细胞侵袭和迁移;MTA1基因可能成为肿瘤治疗上有前途的分子靶点。
Abstract:
Objective To observe the effect of RNAi that silences MTA1 gene on invasion and migration of esophageal carcinoma 9706 cells.  Methods The siRNA expression vector that silences MTA1 gene was transfected into EC9706 cells by liposome. MTA1 mRNA and protein expressions were detected through quantitative RT-PCR and Western blot, respectively. The invasion and migration of EC9706 cells were evaluated by scrape wound healing assay and cell invasion assay in vitroResults MTA1 gene expression significantly decreased. The scrape wound of EC9706 cells healed more slowly and the cell population that cut through Matrigel were less in the EC9706 cells transfected with siRNA expression vector than non-transfected EC9706 cells and the EC9706 cells transfected with blank vector (P<0.05).  Conclusion RNAi silencing MTA1 gene inhibits the invasion and migration of esophageal carcinoma effectively. It is supposed that MTA1 gene may be a prospective molecule target in tumor therapy.

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更新日期/Last Update: 2008-04-07