[1]余维涛,崔社怀,杨雪梅.特异性stealth siRNA抑制小鼠肺成纤维细胞TGF-β1表达的实验研究[J].第三军医大学学报,2007,29(21):2038-2040.
 YU Wei-tao,CUI She-huai,YANG Xue-mei.Inhibitive effect of specific stealth siRNAs on TGF-β1 expression of mouse lung fibroblasts[J].J Third Mil Med Univ,2007,29(21):2038-2040.
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特异性stealth siRNA抑制小鼠肺成纤维细胞TGF-β1表达的实验研究(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
29卷
期数:
2007年第21期
页码:
2038-2040
栏目:
论著
出版日期:
2007-11-15

文章信息/Info

Title:
Inhibitive effect of specific stealth siRNAs on TGF-β1 expression of mouse lung fibroblasts
作者:
余维涛崔社怀杨雪梅
第三军医大学大坪医院野战外科研究所呼吸内科
Author(s):
YU Wei-tao CUI She-huai YANG Xue-mei
Department of Respiratory Diseases, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing 400042, China
关键词:
stealth siRNATGF-β1肺成纤维细胞
Keywords:
stealth siRNA TGF-β1 lung fibroblast
分类号:
R322.35; R329.26; R394.3
文献标志码:
A
摘要:
目的  设计特异性stealth siRNA并检测其对TGF-β1的抑制作用。 方法  针对BALB/c小鼠TGF-β1基因组,选择不同位点设计3套siRNA基因序列,并经化学修饰合成3种stealth siRNA,转染体外培养的小鼠肺成纤维细胞,免疫组化法检测其对TGF-β1表达的影响。  结果  3种stealth siRNA对TGF-β1表达在不同时间有不同程度的抑制作用,以stealth_166效果更为明显;抑制效果与转染时间长短相关,48 h后就可检测出明显抑制,72 h达到最高峰,96 h后开始减弱。  结论  合成的特异性stealth siRNA可以抑制TGF-β1在小鼠肺成纤维细胞的表达,有望成为防治肺间质纤维化的一种手段。
Abstract:
Objective    To design and produce specific stealth siRNAs and detect their inhibitive effects on TGF-β1 expression.     Methods    Three stealth siRNAs aimed directly at different sequences (stealth_48, stealth_166, stealth_594) in TGF-β1 mRNA were made. These siRNAs were transfected into BALB/c mouse lung fibroblast in vitro, then the TGF-β1 expression in those lung fibroblasts was detected by immunohistochemistry.     Results    In different time periods, the TGF-β1 expression was differently depressed by three stealth siRNAs in vitro. The inhibitory effects of stealth_166 was better than the other two stealth siRNAs. The inhibition could be detected in 48 h, reached the highest level in 72 h and began to attenuate 96 h later.     Conclusion    The TGF-β1 expression of mouse lung fibroblasts in vitro can be depressed by synthetic stealth siRNAs. It seems possible to provide a new way for the treatment of pulmonary interstitial fibrosis.

参考文献/References:

余维涛,崔社怀,杨雪梅.  特异性stealth siRNA抑制小鼠肺成纤维细胞TGF-β1表达的实验研究[J]. 第三军医大学学报, 2007, 29(21):2038-2040.

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更新日期/Last Update: 2008-07-02