[1]陈婧,李毅,谭畅,等.TIA-1通过影响肝细胞转录因子的活性调节HBV的e抗原和s抗原表达[J].陆军军医大学学报(原第三军医大学学报),2008,30(09):834-836.
 CHEN Jing,LI Yi,TAN Chang,et al.TIA-1 may interact with liver-enriched transcription factors and regulate HBV s and e antigen expressions[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(09):834-836.
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TIA-1通过影响肝细胞转录因子的活性调节HBV的e抗原和s抗原表达(/HTML )
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陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
30卷
期数:
2008年第09期
页码:
834-836
栏目:
论著
出版日期:
2008-05-15

文章信息/Info

Title:
TIA-1 may interact with liver-enriched transcription factors and regulate HBV s and e antigen expressions
作者:
陈婧李毅谭畅刘湘黄爱龙汤华
重庆医科大学附属第二医院病毒性肝炎研究所,教育部感染性疾病分子生物学重点实验室
Author(s):
CHEN Jing LI Yi TAN Chang LIU Xiang HUANG Ai-long TANG Hua
Key Laboratory of Molecular Biology on Infectious Diseases, Insititute of Viral Hepatitis, The Second Affiliated Hospital of Chongqing Medical University
关键词:
TIA-1s抗原 e抗原转录因子HepG 2.2.15细胞
Keywords:
TIA-1 s antigen e antigen transcription factor HepG 2.2.15 hepatoma cell line
分类号:
R373.21; R392.11; R392.2
文献标志码:
A
摘要:
目的  证明TIA-1分子具有调节HBV基因表达的功能。  方法  TIA-1真核表达质粒pcDNA3-TIA-1,用脂质体转染HepG 2.2.15细胞后,ELISA检测e和s抗原的表达;RT-PCR检测与HBV复制有关的转录因子HNF1、 HNF2、 HNF3、HNF4、 RXRα、 PPARα、C/EBP的表达。  结果  与正常的HepG 2.2.15细胞相比,转染pcDNA3-TIA-1质粒的HepG 2.2.15细胞,其e抗原的表达量明显的增高,而s抗原表达量明显的降低。与正常的HepG 2.2.15细胞相比,转染pcDNA3-TIA-1质粒的HepG 2.2.15细胞中的HNF1、 HNF2、 RXRα mRNA含量有明显的下降。  结论  TIA-1蛋白能通过影响与HBV复制有关的转录因子活性,调节HBV e抗原和s抗原的表达。
Abstract:
Objective  To identify TIA-1 function in regulating HBV replication.   Methods  pcDNA3-TIA-1 eukaryotic expression vector was transfected into HepG 2.2.15 hepatoma cell line with lipofectamine. HBV e antigen and s antigen was checked with ELISA method. RT-PCR was used to identify expression of some transcription factors that involved in HBV transcription such as HNF1, HNF2, HNF3, HNF4, RXRα, PPARα, and C/EBP.   Results  Compared with normal HepG 2.2.15 hepatoma cell, the expression of e antigen was obviously high and s antigen was depressed in the HepG 2.2.15 hepatoma cell transfected with pcDNA3-TIA-1 vector. Compared with normal HepG 2.2.15 hepatoma cell, the mRNA level of HNF1, HNF2, RXRα changed significantly.   Conclusion  These data show that TIA-1 may interact with liver-enriched transcription factors and regulate HBV e antigen and s antigen expression.
更新日期/Last Update: 2008-05-07