[1]陈秀,吴万福,胡长林,等.成年大鼠缺氧性脑损伤后亚临床痫性发作及海马苔藓纤维出芽[J].陆军军医大学学报(原第三军医大学学报),2008,30(01):49-52.
 CHEN Xiu,WU Wan-fu,HU Chang-lin,et al.Subclinical seizure and mossy fiber sprouting in hippocampus of rats following hypoxic brain damage[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(01):49-52.
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成年大鼠缺氧性脑损伤后亚临床痫性发作及海马苔藓纤维出芽(/HTML )
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陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
30卷
期数:
2008年第01期
页码:
49-52
栏目:
论著
出版日期:
2008-01-15

文章信息/Info

Title:
Subclinical seizure and mossy fiber sprouting in hippocampus of rats following hypoxic brain damage
作者:
陈秀吴万福胡长林蔡文琴杨忠
重庆医科大学附属第二医院神经内科;第三军医大学基础医学部神经生物学教研室,重庆市神经科学研究所
Author(s):
CHEN Xiu WU Wan-fu HU Chang-lin CAI Wen-qin YANG Zhong
Department of Neurology, Second Affiliated Hospital of Chongqing Medical University;  Department of Neurobiology, College of Medicine, Third Military Medical University
关键词:
缺氧性脑损伤痫性发作苔藓纤维出芽
Keywords:
hypoxic brain injury seizure mossy fiber sprouting
分类号:
R366.4; R741.02; R742.1
文献标志码:
A
摘要:
目的  探讨缺氧性脑损伤后亚临床痫性发作的病理生理机制。  方法  采用成年雄性SD大鼠,充以8%氧氮混合气体缺氧后,监测大鼠脑电活动,检测海马组织苔藓纤维出芽(mossy fiber sprouting,MFS)的变化。  结果  大鼠缺氧损伤后痫样放电的发生率为19.67% , 亚临床痫性发作组缺氧后7 d海马CA3区下锥体细胞层棕黑色颗粒逐渐增多:14 d呈束状、斑片状颗粒沉积;28 d呈现明显颗粒沉积条带,亚临床痫性发作组海马CA3区苔藓纤维出芽评分明显高于非亚临床痫性发作组和对照组,而3组齿状回(dentate gyrus;DG)内分子层苔藓纤维出芽评分无差异(P>0.05)。  结论  成年大鼠缺氧性脑损害后可出现痫样放电并在海马CA3 区形成苔藓纤维出芽,而海马CA3 区苔藓纤维出芽可能与缺氧性脑损伤后痫性发作有关。
Abstract:
Objective  To explore the pathophysiological mechanism of subclinical seizures following hypoxic brain injury.   Methods  Totally 71 adult male SD rats were set in a close chamber with 8% nitrogen oxygen ventilation (0.5 L/min) till they lost breath every day for 7 d to induce hypoxic brain injury. Another 9 rats living in normal air condition served as control. Electroencephalography was carried out 20 min before the hypoxia and 2 h after hypoxic ventilation to detect subclinical seizures. The animals were sacrificed in 7, 14, and 28 d after the end of hypoxia. Mossy fiber sprouting in the hippocampus were detected by Timm’s histochemical staining.   Results  Subclinical seizures occurred in 19.67% rats (14/71) following hypoxia. In Sub-clinical seizure group, brownish black granule sediment appeared in the pyramidal layer of CA3 region in 7 d following hypoxia, and became more intensive in bundle-like or patch-like way in 14 d, moreover, an obvious stained band was formed in 28 d. Compared with non-subclinical seizure group and control group, the scores of mossy fiber sprouting in the hippocampal CA3 region were higher in subclinical seizure group. But there was no significant difference of mossy fiber sprouting in dentate gyrus among the 3 groups above.   Conclusion Subclinical seizures may occur following hypoxia in adult rats, and prominent mossy fiber spouting is formed in the hippocampal CA3 region of these rats, which may be related to seizure following hypoxic brain damage .

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[2]陈秀,吴万福,胡长林,等.缺氧性脑损伤对大鼠癫痫敏感性和脑内PSD-95表达的影响[J].陆军军医大学学报(原第三军医大学学报),2008,30(14):1353.
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更新日期/Last Update: 2008-05-05