[1]矫树生,李兵仓,陈建梅,等.嗅鞘细胞与聚乳酸-聚羟基乙酸共聚物材料的细胞相容性研究[J].陆军军医大学学报(原第三军医大学学报),2008,30(05):370-373.
 JIAO Shu-sheng,LI Bing-cang,CHEN Jian-mei,et al.Cytocompatibility of biomaterial poly (DL-lactide-co-glycolide) with olfactory ensheathing cells[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(05):370-373.
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嗅鞘细胞与聚乳酸-聚羟基乙酸共聚物材料的细胞相容性研究(/HTML )
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陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
30卷
期数:
2008年第05期
页码:
370-373
栏目:
论著
出版日期:
2008-03-15

文章信息/Info

Title:
Cytocompatibility of biomaterial poly (DL-lactide-co-glycolide) with olfactory ensheathing cells
作者:
矫树生李兵仓陈建梅游华
第三军医大学大坪医院野战外科研究所第六研究室,创伤、烧伤与复合伤国家重点实验室; 南京军区福州总医院骨科
Author(s):
JIAO Shu-sheng LI Bing-cang CHEN Jian-mei YOU Hua
State Key Laboratory of Trauma, Burn and Combined Injury, Department of 6, Research Institute of Surgery, Daping Hospital, Third Military Medical University; Fuzhou General Hospital of Nanjing Command
关键词:
嗅鞘细胞聚乳酸聚羟基乙酸共聚物细胞相容性人工神经
Keywords:
olfactory ensheathing cells poly (DL-lactide-co-glycolide) cytocompatibility artificial nerve
分类号:
R318.08; R322.8; R329.24
文献标志码:
A
摘要:
目的 探讨嗅鞘细胞(olfactory ensheathing cells, OECs)与聚乳酸聚羟基乙酸共聚物[poly(DL-lactide-co-glycolide),PLGA]材料的细胞相容性。 方法 将新鲜分离的OECs细胞悬液接种于PLGA膜上,然后用倒置相差显微镜、扫描电镜观察细胞生长及细胞与材料的附着情况;MTT、流式细胞术等方法测定OECs的细胞增殖和细胞周期变化。 结果 PLGA膜上培养OECs的形态特征、增殖能力、细胞周期及倍体水平等与正常培养的OECs相比,均无显著性差异(P>0.05)。 结论 PLGA生物材料与OECs相容性好,适于构建组织工程化人工神经。
Abstract:
Objective To investigate the cytocompatibility between poly (DL-lactide-co-glycolide) (PLGA) and olfactory ensheathing cells (OECs) so as to found a basis to prepare an artificial nerve made of PLGA with OECs.  Methods Freshly isolated OECs were seeded on PLGA membrane, and then their growth and adhesion to the membrane were observed at different time points by phase contrast inverted microscopy and scanning electron microscopy. The proliferation and the cell cycle of OECs were examined by MTT and flow cytometry.   Results There was no statistically difference between the morphological feature, proliferative capacity and cell cycle of OECs cultured on PLGA membrane and those normally cultured (P>0.05).   Conclusion The PLGA processes good compatibility with OECs, and it is suitable to construct tissue engineering artificial nerve with OECs.

相似文献/References:

[1]梁涛,李淑蓉,邓其跃,等.NOV在嗅鞘细胞中的表达及活性检测[J].陆军军医大学学报(原第三军医大学学报),2005,27(07):596.
[2]谢晶,李瑶琛,阴正勤.嗅鞘细胞移植后通过分泌MMP-3在RCS-P+大鼠视网膜中迁移[J].陆军军医大学学报(原第三军医大学学报),2012,34(09):843.
 Xie Jing,Li Yaochen,Yin Zhengqin.Transplanted olfactory ensheathing cells migrate in RCS-P+ rat retina through secreting MMP-3[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(05):843.

更新日期/Last Update: 2008-04-21