[1]干晓琴,姚珍薇.CO2气腹对人宫颈癌CaSki细胞Survivin和Bcl-2表达的影响[J].第三军医大学学报,2007,29(13):1323-1325.
 GAN Xiao-qin,YAO Zhen-wei.Effect of imitated CO2 pneumoperitoneum on expressions of Survivin and Bcl-2 in human cervical carcinoma cell line[J].J Third Mil Med Univ,2007,29(13):1323-1325.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
29卷
期数:
2007年第13期
页码:
1323-1325
栏目:
论著
出版日期:
2007-07-15

文章信息/Info

Title:
Effect of imitated CO2 pneumoperitoneum on expressions of Survivin and Bcl-2 in human cervical carcinoma cell line
作者:
干晓琴 姚珍薇
重庆医科大学附属第一医院妇产科
Author(s):
GAN Xiao-qin YAO Zhen-wei
Department of Obstetrics and Gynecology, The First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China
关键词:
二氧化碳气腹宫颈肿瘤SurvivinBcl-2
Keywords:
carbon dioxide pneumoperitoneum cervical neoplasm Survivin Bcl-2
分类号:
R363.13; R730.23; R737.33
文献标志码:
A
摘要:
目的    探讨CO2气腹对人宫颈癌CaSki细胞抗凋亡能力的影响。    方法    建立腹腔镜CO2气腹体外模型,以不同压力(0、7、14 mmHg)的CO2气腹对人宫颈癌CaSki细胞处理不同时间(1、2、3、4 h),设未处理为对照组。采用RT-PCR、Western blot分别检测CO2气腹处理后各组细胞Survivin mRNA 和Bcl-2蛋白的表达情况。    结果    当压力为7、14 mmHg时,Survivin和Bcl-2的表达均随着气腹处理时间的延长呈上升趋势(P<0.05)。当处理相同时间时,7 mmHg组Survivin和Bcl-2的表达量均明显高于其他压力组和对照组(P<0.05)。    结论     在一定压力和作用时间范围,CO2气腹能提高宫颈癌细胞的抗凋亡能力,这可能是CO2气腹促肿瘤生长的机制之一。
Abstract:
Objective    To study the effect of CO2 pneumoperitoneum on the anti-apoptotic capability of human cervical carcinoma cell line (CaSki).       Methods    The imitated CO2 pneumoperitoneum was established in vitro. After CaSki cells were exposed to CO2 pneumoperitoneum at 0, 7 or 14 mmHg for 1, 2, 3, 4 h, the expressions of Survivin mRNA and Bcl-2 protein were examined by RT-PCR and Western blot respectively. The untreated CaSki cells served as control.      Results    Under 7 or 14 mmHg, the expressions of Survivn and Bcl-2 had an increasing tendency with the exposure time prolongation. Within the same exposure time, the expressions of Survivin and Bcl-2 under 7 mmHg were significantly higher than those under 0 or 14 mmHg or in control group.     Conclusion    Under certain pressure and within certain exposure time, CO2  pneumoperitoneum could improve anti-apoptotic capability of human cervical carcinoma cells, which may be one reason for the promoting effect of carbon dioxide pneumoperitoneum on malignant cells growth.

参考文献/References:

干晓琴, 姚珍薇. CO2气腹对人宫颈癌CaSki细胞Survivin和Bcl-2表达的影响[J]. 第三军医大学学报, 2007, 29(13):1323-1325.

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更新日期/Last Update: 2008-10-15