[1]耿力,姚珍薇,乐爱文,等.绝经后女性雌激素受体β基因多态性与骨密度的关系[J].第三军医大学学报,2008,30(04):333-336.
 GENG Li,YAO Zhen-wei,LE Ai-wen,et al.Association of polymorphism of estrogen receptor β gene with bone mineral density after menopause[J].J Third Mil Med Univ,2008,30(04):333-336.
点击复制

绝经后女性雌激素受体β基因多态性与骨密度的关系(/HTML )
分享到:

《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
30卷
期数:
2008年第04期
页码:
333-336
栏目:
论著
出版日期:
2008-02-28

文章信息/Info

Title:
Association of polymorphism of estrogen receptor β gene with bone mineral density after menopause
作者:
耿力姚珍薇乐爱文骆建云韩力力卢起
重庆医科大学附属第一医院妇产科
Author(s):
GENG Li YAO Zhen-wei LE Ai-wen LUO Jian-yun HAN Li-li LU Qi
Department of Obstetrics and Gynecology, The First Affiliated Hospital, Chongqing Medical University
关键词:
雌激素受体β基因遗传多态性骨密度骨质疏松症
Keywords:
estrogen receptor β gene genetic polymorphism bone mineral density osteoporosis
分类号:
R322.71;R394.1;R711.510.2
文献标志码:
A
摘要:
目的 探讨绝经后女性雌激素受体β基因(estrogen receptor β, ESR2)CA重复序列多态性及G1082A多态性与骨密度的关系。   方法 随机数字表法抽取重庆地区绝经后女性200例,采用基因扫描及DNA测序技术分析ESR2基因CA重复序列多态性,采用多聚酶链反应限制性片段长度多态性法分析ESR2基因G1082A多态性,使用双能X线骨密度仪测量股骨近端及腰椎骨密度。   结果 以CA重复序列平均数22次为界,将重复序列基因分为短基因(<22)和长基因(≥22),分别以S和L表示。200名绝经后女性中CA重复序列基因型SS、SL及LL的频率分别为25.0%、46.0%及290%;G1082A基因型rr、Rr及RR的频率分别为43.0%、42.0%及15.0%;基因型频率分布均符合HardyWeinberg平衡(P>0.05)。在校正年龄、绝经年限、身高、体质量和体质指数后,CA重复序列SS基因型者股骨颈骨密度较SL或LL基因型者高(P<0.01),其余部位(大转子、 Wards三角和腰椎)各基因型骨密度无统计学差异(P>0.05);G1082A位点各基因型股骨颈、大转子、Wards三角及腰椎骨密度无统计学差异(P>0.05);这2个ESR2基因的多态性位点非连锁不平衡(P>0.05)。   结论 绝经女性中,ESR2基因第5内含子的CA重复序列多态性可能对骨密度的遗传调节起作用,而ESR2基因第5外显子的G1082A多态性可能对骨密度无明显影响。
Abstract:
Objective To investigate the association of CA repeat polymorphism and G1082A polymorphism of the estrogen receptor β (ESR2) gene with bone mineral density (BMD) in postmenopausal women.   Methods Two hundred postmenopausal women who visited our outpatient clinic were enrolled. The CA repeat polymorphism of ESR2 gene was determined by gene scan and DNA sequence methods. The G1082A polymorphism of ESR2 gene was detected by polymerase chain reactionrestriction fragment length polymorphism. BMD for the proximal femur and lumbar spine (L2-L4) was measured by dual-energy X-ray absorptiometry.   Results The  (CA)n<22 and (CA)n≥22 alleles were designated as S and L, respectively. The frequency of genotypes in 200 postmenopausal women was as follows: SS (25.0%), SL (46.0%), LL (29.0%) for CA repeat polymorphism, and rr (43.0%), Rr (42.0%), RR (15.0%) for G1082A polymorphism. The distribution of genotypes was in Hardy-Weinberg equilibrium. After adjusting for age, menopausal period, height, weight and body mass index, BMD was higher in postmenopausal women with SS genotype than SL or LL genotype for CA repeat polymorphism at femoral neck (P<0.01), but there was no significant difference in BMD of trochanter, Wards triangle and lumbar spine for different genotypes (P>0.05). In different genotypes for G1082A polymorphism, there was no significant difference in BMD of each skeletal region (P>0.05). The ESR2 polymorphism was not in linkage disequilibrium (P>0.05).  Conclusion In postmenopausal women, CA repeat polymorphism in the fifth intron of the ESR2 gene may contribute to the genetic regulation of BMD, but the G1082A polymorphism in the fifth exon of the ESR2 gene may not significantly affect BMD.

参考文献/References:

耿力, 姚珍薇, 乐爱文,等. 绝经后女性雌激素受体β基因多态性与骨密度的关系[J].第三军医大学学报,2008,30(4):333-336.

相似文献/References:

[1]苟本富,魏泓.南江黄羊基因组DNA的AFLP和RAPD研究[J].第三军医大学学报,2005,27(17):1778.
[2]耿力,杨洪昌,陈仲,等.CYP19基因多态性与绝经后骨质疏松症的关联性[J].第三军医大学学报,2010,32(14):1534.
 Geng Li,Yang Hongchang,Chen Zhong,et al.Correlation of CYP19 gene polymorphisms with postmenopausal osteoporosis in Chongqing Chinese Han women[J].J Third Mil Med Univ,2010,32(04):1534.
[3]何谐,张锡林,牛靖萱,等.日本血吸虫和斯氏并殖吸虫的rDNA-ITS2遗传多态性研究[J].第三军医大学学报,2009,31(21):2073.
 HE Xie,ZHANG Xi-lin,NIU Jing-xuan,et al.Genetic polymorphism of rDNA-ITS2 of Schistosoma japonicum and Paragonimus skrjabini[J].J Third Mil Med Univ,2009,31(04):2073.
[4]梁智勇,史景泉,魏泓,等.10个品系小鼠的AFLP分析[J].第三军医大学学报,2003,25(02):0.[doi:10.16016/j.1000-5404.2003.02.027 ]
 LIANG Zhi yong,SHI Jing quan,WEI Hong,et al.[J].J Third Mil Med Univ,2003,25(04):0.[doi:10.16016/j.1000-5404.2003.02.027 ]

更新日期/Last Update: 2008-04-29