[1]郭树章,任先军,蒋涛,等.脱细胞脊髓支架的成分分析[J].陆军军医大学学报(原第三军医大学学报),2007,29(13):1313-1315.
　GUO Shu-zhang,REN Xian-jun,JIANG Tao,et al.Componential analysis of acellular scaffold of spinal cord[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(13):1313-1315.
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陆军军医大学学报（原第三军医大学学报）[ISSN:1000-5404/CN:51-1095/R]

卷:
29卷
期数:
2007年第13期
页码:
1313-1315
栏目:
论著
出版日期:
2007-07-15

文章信息/Info

Title:
Componential analysis of acellular scaffold of spinal cord
作者:
郭树章; 任先军; 蒋涛; 吴波
第三军医大学新桥医院骨科
Author(s):
GUO Shu-zhang;  REN Xian-jun;  JIANG Tao;  WU Bo
Department of Orthopaedics, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China
关键词:
脱细胞脊髓; 组织工程支架; 成分; 大鼠
Keywords:
acellular spinal cord;  tissue engineering scaffold;  component;  rat
分类号:
R318.08; R322.81
文献标志码:
A
摘要:
目的    探讨同种异体脱细胞脊髓支架的制备方法并分析其可能含有的有效成分。    方法    采用冻融+化学萃取方法制备脱细胞脊髓支架，光镜及扫描电镜观察其结构特征；用免疫组织化学法分析脱细胞支架的主要成分。    结果    正常的大鼠脊髓组织经脱细胞处理后，清除了所有的细胞成分及髓鞘和轴突，扫描电镜显示保存了细胞外基质成分构成了三维支架结构。成分分析结果显示，存留的支架结构中含有层黏连蛋白（laminin, LN）、纤连蛋白（fibronectin, FN）、Ⅳ型胶原等诱导和促进神经再生、促进细胞黏附和增殖的重要成分。    结论    脱细胞异体脊髓具有三维支架结构，含有诱导和促进神经再生的相关蛋白，有利于受损神经的再生及移植细胞的存活和增殖。
Abstract:
Objective    To prepare the acellular scaffold of spinal cord and analyze its component.     Methods    The acellular scaffold was prepared with the freeze thawing and chemical extraction, its structure was observed by HE and SEM, its component was analyzed by immunohistochemistry.     Results    The cells, myelin sheath and axon of nerve fibers in the rat spinal cord were eliminated, but three-dimensional supports of extracellular matrix were reserved. The analytical results showed the component of the acellular spinal cord contain laminin, fibronectin and type Ⅳ collagen—the necessary components to facilitate and induce the regeneration of the injured nerves and enhance the adhesion and proliferation of cells.     Conclusion    The acellular spinal cord has three dimensional structure and contains several proteins related to the regeneration of the injured nerves and promotion of  the survival and proliferation of cells.

相似文献/References:

[1]蒋涛,任先军,阴洪,等.京尼平交联对大鼠脱细胞脊髓生物学特性的影响[J].陆军军医大学学报(原第三军医大学学报),2013,35(20):2164.
　Jiang Tao,Ren Xianjun,Yin Hong,et al.Effect of genipin cross-linking on biological characteristics of rat acellular spinal cord[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(13):2164.
[2]郭树章,蒋涛,任先军.2种去细胞方法制备大鼠脱细胞脊髓支架效果的对比研究[J].陆军军医大学学报(原第三军医大学学报),2010,32(01):19.
　Guo Shuzhang,Jiang Tao,Ren Xianjun.Comparison of 2 decellularizing methods to prepare rat acelluar allograft spinal cord scaffold[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(13):19.

更新日期/Last Update: 2008-10-15