[1]王庆红,王晓娟,甘成军,等.TGF-β1在ConA激活的CD4+T细胞抑制NK细胞杀伤毒性中的影响[J].陆军军医大学学报(原第三军医大学学报),2007,29(22):2145-2147.
 WANG Qing-hong,WANG Xiao-juan,GAN Cheng-jun,et al.Cytotoxicity of NK cells inhibited by CD4+T cells stimulated by ConA[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(22):2145-2147.
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TGF-β1在ConA激活的CD4+T细胞抑制NK细胞杀伤毒性中的影响(/HTML )
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陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
29卷
期数:
2007年第22期
页码:
2145-2147
栏目:
论著
出版日期:
2007-11-30

文章信息/Info

Title:
Cytotoxicity of NK cells inhibited by CD4+T cells stimulated by ConA
作者:
王庆红王晓娟甘成军彭旭罗高兴吴军
第三军医大学西南医院全军烧伤研究所,创伤、烧伤与复合伤国家重点实验室, 疾病蛋白质组学重庆市市级重点实验室
Author(s):
WANG Qing-hong WANG Xiao-juan GAN Cheng-jun PENG Xu LUO Gao-xing WU Jun
State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Burns, Chongqing Key Laboratory for Proteomics of Diseases, Southwest Hospital, Third Military Medical University, Chongqing 400038, China
关键词:
CD4+-T细胞NK细胞TGF-β1 抗体细胞毒性
Keywords:
CD4+T cells NK cells TGF-β1antibody cytotoxicity
分类号:
R392-33;R392.12
文献标志码:
A
摘要:
目的  初步探讨ConA激活的CD4+T细胞对NK细胞杀伤毒性的影响及TGF-β1分子在其中的作用。  方法  磁珠分选纯化BALB/c小鼠脾脏CD4+T细胞与NK细胞,体外用ConA激活CD4+T细胞,将激活的CD4+T细胞与NK细胞共培养,并加入TGF-β1抗体,共培养24、48、72 h和96 h后,用51Cr标记的YAC-1检测NK细胞的杀伤毒性。  结果 ConA 激活的CD4+T细胞能够在所测的4个时相点显著抑制NK细胞的杀伤毒性,抑制率分别为25.00%、31.60%、34.70%和46.50%;在此共培养体系中加入TGF-β1抗体后,抑制率分别增加到63.80%、59.43%、60.70%和90.90%。    结论 ConA 激活的CD4+T细胞能够显著抑制NK细胞的杀伤毒性,并且这种抑制作用随着TGF-β1抗体的加入而增强,表明TGF-β1分子影响了ConA激活的CD4+T细胞对NK细胞杀伤毒性的抑制。
Abstract:
Objective    To explore the effect of CD4+T cells stimulated by ConA on the cytotoxic activity of NK cells.     Methods    CD4+T cells and NK cells were purified from spleen of BALB/c mice spleen by magnetic cell sorting. CD4+T cells stimulated by ConA and NK cells were co-cultured with or without anti-TGF-β1 antibody in the co-culture system. The cytotoxicity of NK cells was assayed by YAC-1 labeled by 51Cr in 24, 48, 72 and 96 h.     Results    The cytotoxicity of NK cells were inhibited significantly by CD4+T cells activated by ConA at 4 time points and the inhibitory rate respectively was 25%, 31.6%, 34.7% and 46.5%. After anti-TGF-β1 antibody was added into the co-culture system, the inhibitory rate reached to 63.8%, 59.43%, 607% and 90.9%.       Conclusion   In vitro, CD4+ T cells stimulated by ConA notably inhibits the cytotoxicity of NK cells, and TGF-β1 pathway may be involved in this action.

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更新日期/Last Update: 2008-07-01