[1]陈健,李大江,王曙光.腺病毒介导凋亡素基因对裸鼠种植性人胆管癌的治疗研究[J].第三军医大学学报,2007,29(11):1036-1038.
 CHEN Jian,LI Da-jiang,WANG Shu-guang.Therapy for xenograft human bile duct cancer in mice mediated by adenovirus containing Apoptin gene[J].J Third Mil Med Univ,2007,29(11):1036-1038.
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腺病毒介导凋亡素基因对裸鼠种植性人胆管癌的治疗研究
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
29卷
期数:
2007年第11期
页码:
1036-1038
栏目:
论著
出版日期:
2007-06-15

文章信息/Info

Title:
Therapy for xenograft human bile duct cancer in mice mediated by adenovirus containing Apoptin gene
作者:
陈健 李大江 王曙光
第三军医大学西南医院全军肝胆外科研究所
Author(s):
CHEN Jian LI Da-jiang WANG Shu-guang
Institute of Hepatobiliary Surgery, Southwest Hospital, Third Military Medical University, Chongqing 400038, China
关键词:
凋亡素基因腺病毒载体基因治疗人胆管癌
Keywords:
Apoptin gene  adenovirus vector  gene therapy  human bile duct cancer
分类号:
R73-354; R730.59; R735.8
文献标志码:
A
摘要:
目的     探讨腺病毒介导凋亡素基因对裸鼠种植性人胆管癌的治疗。     方法    建立裸鼠皮下种植性人胆管癌模型,观察肿瘤内注射含凋亡素基因的重组腺病毒后种植瘤的体积变化、毒副作用和组织学变化,同时计算抑瘤率。    结果    在治疗12 d后含凋亡素基因的腺病毒治疗组的种植瘤体积为(92.31±28.31)mm,较空病毒组(288.86±113.13)mm和生理盐水组(344.86±113.87)mm相比具有显著差别(P<0.01)。同时抑瘤率达到72.10%,明显高于空病毒治疗组11.9%(P<0.01)。在治疗期间没有观察到毒副作用。组织学结果也提示凋亡素基因是通过引发胆管癌细胞的凋亡来抑制肿瘤生长的。    结论    含凋亡素基因的重组腺病毒对胆管癌具有治疗作用。
Abstract:
Objective    To study the therapy for xenograft human bile duct cancer in mice mediated by adenovirus containing Apoptin gene.     Methods    Subcutaneous human bile duct cancer was established in nude mice. Variations of tumor volume and histomorphology, side effects were observed after intratumoral injection of adenovirus containing Apoptin gene. Finally the mice were sacrificed for calculating the ratio of antitumor.     Results    Twelve days after treatment, the mean volume of the xenograft human bile duct cancer in the group of intratumoral injection of adenovirus containing Apoptin gene was (92.31±28.31) mm, which was reduced significantly compared with that of adenovirus infection without apoptin gene(288.86±113.13)mm and control group(344.86±113.87)mm. The ratio of antitumor was 72.10%, which was significantly higher than that in control group(11.9%). During the whole experimental course, no side effect was observed. The histological results demonstrated that the reduction of tumor growth was the result of apoptosis in bile duct cells, which was reduced by transfection of Apoptin gene.     Conclusion    The adenovirus vectors containing Apoptin gene may constitute a safe tool for the treatment of cholangiocarcinoma.

参考文献/References:

陈健, 李大江, 王曙光. 腺病毒介导凋亡素基因对裸鼠种植性人胆管癌的治疗研究[J]. 第三军医大学学报, 2007, 29(11):1036-1038.

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更新日期/Last Update: 2008-10-23