[1]吴跃,苟欣,吕天兵,等.3种不同的survivin siRNA对EJ28细胞增殖和凋亡的影响[J].第三军医大学学报,2007,29(22):2152-2155.
 WU Yue,GOU Xin,LU Tian-bing,et al.Proliferation and apoptosis of EJ28 cells induced by 3 different survivin-targeted siRNA[J].J Third Mil Med Univ,2007,29(22):2152-2155.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
29卷
期数:
2007年第22期
页码:
2152-2155
栏目:
论著
出版日期:
2007-11-30

文章信息/Info

Title:
Proliferation and apoptosis of EJ28 cells induced by 3 different survivin-targeted siRNA
作者:
吴跃苟欣吕天兵余周
重庆医科大学附属第一医院泌尿外科
Author(s):
WU Yue GOU XinLU Tian-bing YU Zhou
Department of Urology, the First affiliated Hospital of Chongqing Medical University, Chongqing 400016, China
关键词:
EJ28细胞survivin小干扰RNA转染
Keywords:
EJ28 cellssurvivin small interfering RNAtransfection
分类号:
R73-362;R730.23;R737.14
文献标志码:
A
摘要:
目的  观察靶向survivin的不同siRNA对EJ28细胞增殖和凋亡的影响。  方法  通过化学合成方法合成3对siRNA和1对荧光标记的阴性对照siRNA。转染荧光标记的siRNA后,在荧光显微镜下观察转染效率。实验分为siRNA164、siRNA167、siRNA389、阴性对照组、脂质体对照组和细胞对照组。转染后24、48 h和72 h,MTT法检测siRNA对EJ28细胞增殖的影响,流式细胞术检测凋亡率。  结果  靶向survivin的序列特异性siRNA均能抑制EJ28细胞的增殖,  其中以siRNA164抑制细胞的增殖能力最强(P<0.05),转染48 h后的抑制率最高[(41.32±2.54)%];转染48 h后,siRNA164 组凋亡率最高[(13.20±0.25)%]。  结论  靶向survivin的RNAi技术在膀胱癌的基因治疗中可能具有一定的价值。
Abstract:
Objective    To explore the proliferative and apoptotic effect of 3 different siRNAs targeting survivin in human bladder cancer cell line EJ28.     Methods    Three siRNAs targeting survivin and 1 fluorescence-labeled siRNA as a negative control were chemically synthesized. Transfetion efficiency was observed under fluorescence microscope after transfecting the fluorescence-labeled siRNAs. EJ28 cells were divided into 6 groups: siRNA164 group, siRNA167 group, siRNA389 group, and negative control group, lipofectin group and cell control group. The proliferation of EJ28 cell was detected by MTT assay at 24, 48 and 72 h after the transfection. Apoptotic rate was detected by Annexin V-FITC-FCM assay at 48 h.    Results    All the sequence-specific siRNAs targeting survivin efficiently suppressed the proliferation of EJ28 cells. siRNA 164 had the most powerful effect because of its highest inhibitory rate of (41.32±2.54)% and the highest apoptotic rate of (13.20±0.25)% at 48 h after transfection.     Conclusion    RNAi targeting survivin has a potential value in the gene therapy of bladder cancer.

参考文献/References:

吴跃,苟欣,吕天兵,等.  3种不同的survivin siRNA对EJ28细胞增殖和凋亡的影响[J]. 第三军医大学学报, 2007, 29(22):2152-2155.

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更新日期/Last Update: 2008-07-01