[1]武强,李露斯,范文辉,等.小鼠胚胎神经干细胞海马移植对APP/PS1双转基因AD小鼠的治疗作用[J].陆军军医大学学报(原第三军医大学学报),2007,29(10):915-918.
 WU Qiang,LI Lu-si,FAN Wen-hui,et al.Therapeutic effect of mouse embryonic neural stem cells replacement into hippocampus of APP/PS1 double transgenic mice of Alzheimer disease[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(10):915-918.
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小鼠胚胎神经干细胞海马移植对APP/PS1双转基因AD小鼠的治疗作用
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陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
29卷
期数:
2007年第10期
页码:
915-918
栏目:
论著
出版日期:
2007-05-30

文章信息/Info

Title:
Therapeutic effect of mouse embryonic neural stem cells replacement into hippocampus of APP/PS1 double transgenic mice of Alzheimer disease
作者:
武强李露斯范文辉宋敏杨忠
第三军医大学:西南医院神经内科,基础医学部医学遗传学教研室,基础医学部神经生物学教研室,重庆市神经科学研究所
Author(s):
WU Qiang LI Lu-si FAN Wen-hui SONG Min YANG Zhong
Department of Neurology, Southwest Hospital, Department of Medical Genetics, Department of Neurobiology, College of Medicine, Third Military Medical University, Chongqing 400038, China
关键词:
阿尔茨海默病胚胎神经干细胞转基因小鼠移植
Keywords:
Alzheimer disease embryonic neural stem cells transgenic mice transplantation
分类号:
R329.2;R651.11;R749.160.5
文献标志码:
A
摘要:
目的    在APP/PS1双转基因阿尔茨海默病(Alzheimer disease, AD)小鼠观察神经干细胞(nerual stem cells, NSCs)移植后细胞的存活、迁移以及对小鼠记忆功能的影响。    方法    增强型绿色荧光蛋白(enhanced green fluorescent protein, EGFP)质粒转染培养胚胎NSCs,小鼠海马内移植,水迷宫实验检测小鼠认知功能。    结果    EGFP转染NSCs海马内移植2个月后可观察到GFP阳性细胞,大部分分布在针道附近,部分向同侧皮层迁移,亦有部分通过胼胝体向对侧大脑迁移,同时小量细胞发出类似于神经元的长突起。AD小鼠的记忆功能明显改善。    结论    胚胎NSCs海马内移植后能存活、迁移并分化为神经组织细胞,并能显著改善APP/PS1双转基因AD小鼠的认知功能障碍。
Abstract:
Objective    To observe the survival, migration and effect of mouse embryonic neural stem cells (NSCs) after NSCs transplantation into the hippocampus of APP/PS1 double transgenic mice of Alzheimer disease (AD).     Methods    EGFP gene was transfered into NSCs by NucleofectorTM. The memory of mice was evaluated by Morris water maze test after NSCs transplantation into the hippocampus.     Results    Two months after NSCs transplantation, most of the GFP positive cells were found near the pin hole, and some migrated into the ipsilateral cortex as well as opposite side cortex through corpus callosum. Long processes were observed in a few positive cells. Hidden platform test showed the mean latency in transgenic mice transplanted with NSCs was markedly shortened.     Conclusion    Transplanted NSCs can survive, migrate and differentiate into nerve cells in the hippocampus of APP/PS1 double transgenic mice. The memory impairment of APP/PS1 double transgenic mice of Alzheimer disease was markedly relieved.

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更新日期/Last Update: 2008-10-24