[1]易龙,张乾勇,糜漫天,等.ω-3多不饱和脂肪酸抑制人前列腺癌细胞黏附和侵袭的体外实验研究[J].陆军军医大学学报(原第三军医大学学报),2007,29(05):396-398.
 YI Long,ZHANG Qian-yong,MI Man-tian,et al.Effects of ω-3 polyunsaturated fatty acid on adhesion and invasion of human prostate cancer PC-3 cells in vitro[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(05):396-398.
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ω-3多不饱和脂肪酸抑制人前列腺癌细胞黏附和侵袭的体外实验研究(/HTML )
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陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
29卷
期数:
2007年第05期
页码:
396-398
栏目:
论著
出版日期:
2007-03-15

文章信息/Info

Title:
Effects of ω-3 polyunsaturated fatty acid on adhesion and invasion of human prostate cancer PC-3 cells in vitro
作者:
易龙张乾勇糜漫天韦娜郎海滨
第三军医大学军事预防医学院营养与食品卫生学教研室,重庆市营养与食品安全重点实验室
Author(s):
YI LongZHANG Qian-yongMI Man-tianWEI-NaLANG Hai-bin
Department of Nutrition and Food Hygiene, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China
关键词:
PC-3细胞黏附侵袭ω-3多不饱和脂肪酸转移
Keywords:
PC-3 cellsadhesioninvasionω-3 polyunsaturated fatty acidmetastasis
分类号:
R73-37;R737.25;R977.6
文献标志码:
A
摘要:
目的     观察两种ω-3多不饱和脂肪酸(ω-3 polyunsaturated fatty acid, ω-3 PUFA)二十碳五烯酸(eicosapentaenoic acid,EPA)和二十二碳六烯酸(docosahexaenoic acid,DHA)对高转移性人前列腺癌细胞PC-3体外黏附和侵袭能力的影响,并探讨其相关分子机制。      方法     MTT法观察EPA和DHA对PC-3细胞增殖的影响;以体外黏附和侵袭实验观察EPA和DHA对肿瘤细胞黏附和侵袭能力的影响;RT-PCR法观察EPA和DHA对与细胞骨架相关的RhoA、Rac1、Rac2和Cdc42基因mRNA表达的影响。     结果     EPA及DHA均能抑制PC-3细胞的增殖,并呈剂量效应关系;PC-3细胞以60 μmol/L EPA和DHA分别处理后,体外黏附和侵袭能力均降低,在matrigel基质胶上黏附30、60、90 min和120 min的黏附率均低于对照组(P<0.05,P<0.01),侵袭18 h后的抑制率分别为(35.65±7.76)%和(41.32±5.86)%(P<0.01),RhoA、Rac2和Cdc42基因mRNA表达均不同程度下降。     结论     ω-3 PUFA可能通过下调Rho GTP酶mRNA的表达,抑制PC-3细胞体外黏附和侵袭能力。
Abstract:
Objective     To investigate the effects of 2 kinds of ω-3 polyunsaturated fatty acid, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), on the adhesion and invasion of PC-3, a high metastatic human prostate tumor cell line, and explore the possible mechanism.      Methods     MTT assay was used to determine the effects of EPA or DHA on the proliferation of PC-3 cells. Adhesion assay and invasion assay were used to observe the anti-adhesion and anti-invasion effects of EPA or DHA on PC-3 cells, respectively. RT-PCR analysis was conducted to detect the expression of RhoA, Rac1, Rac2 and Cdc42 mRNAs.       Results     Both EPA and DHA inhibited the growth of PC-3 cells in a dose-dependent manner. After treated with EPA or DHA of 60 μmol/L, the adhesion and invasion abilities of the cells were decreased. The adhesion rate were lesser than that of the control when adhered for 30, 60, 90 and 120 min on matrigel, and the invasion inhibitory rate were (35.65±7.76)% in EPA treated group and (41.32±5.86)% in DHA treated group (P<0.01). The expressions of RhoA, Rac2 and Cdc42 in PC-3 cells were all decreased in compare with those of the control.      Conclusion     These results suggest that ω-3 PUFA can inhibit the adhesion and invasion of PC-3 cells by down-regulating the expression of the Rho GTPase.

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更新日期/Last Update: 2008-10-30