[1]郭刚,童文德,邹全明,等.HP0318基因在幽门螺杆菌适应性定植中的作用研究[J].陆军军医大学学报(原第三军医大学学报),2007,29(11):1006-1009.
 GUO Gang,TONG Wen-de,ZOU Quan-ming,et al.Role of HP0318 gene for adaptive colonization of H. pylori in Mongolian gerbils[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(11):1006-1009.
点击复制

HP0318基因在幽门螺杆菌适应性定植中的作用研究
(/HTML )
分享到:

陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
29卷
期数:
2007年第11期
页码:
1006-1009
栏目:
论著
出版日期:
2007-06-15

文章信息/Info

Title:
Role of HP0318 gene for adaptive colonization of H. pylori in Mongolian gerbils
作者:
郭刚童文德邹全明吴超刘开云
第三军医大学医学检验系临床微生物学及免疫学教研室
Author(s):
GUO Gang TONG Wen-de ZOU Quan-ming WU Chao LIU Kai-yun
Department of Clinical Microbiology, College of Medical Laboratory, Third Military Medical University, Chongqing 400038,  China
关键词:
幽门螺杆菌HP0318同源重组突变体
Keywords:
Helicobacter pylori HP0318 homologous recombination mutant
分类号:
R378.99;R394-33; R394.3
文献标志码:
A
摘要:
目的    通过构建幽门螺杆菌(Hp) HP0318缺失突变株,初步探明该基因在Hp适应性定植中的作用。    方法     通过基因克隆和重组技术在HP0318基因上下游片段之间连入氯霉素抗性基因(catGC)作为筛选标记,并克隆到质粒pBluescript SK中构建成缺失突变的打靶载体,将其转化沙鼠适应株M13,经过同源重组,对出发菌的HP0318基因进行置换突变,然后用经氯霉素抗性筛选的突变菌株感染蒙古沙鼠,观察其与未突变的M13出发菌在定植能力和致病性上的差异。    结果    经PCR鉴定成功构建1株缺失HP0318基因的Hp菌株。突变株与非突变株对蒙古沙鼠的感染率相差显著(P<0.05),2个菌株在胃内的定植密度有显著性差异(P<0.05),突变株定植密度有约5倍的减少。    结论    HP0318基因在Hp的沙鼠适应性定植过程中发挥了重要的作用。
Abstract:
Objective    To construct and identify a deficiency mutant of H. pylori strains without HP0318 gene.     Methods    Two cloning PCR fragments containing two-end regions of HP0318 and a selectable chloramphenicol resistance marker between them were engineered into pBluescript plasmid. Then the HP0318-deleted derivative plasmid was electrotransformed into adaptive strain M13 and constructed a HP0318-negative mutant of H. pylori via homologous recombination resulting in allelic exchange. M13 and the mutant were inoculated to Mongolian gerbils, respectively. The level of colonization and pathological change were assessed at 4 weeks after infection.     Results    An HP0318 negative mutant of H. pylori strain has been constructed successfully. The infectious rate and colonization levels of the HP0318 mutant in Mongolian gerbils were significantly lower as compared with M13 (P<0.05).     Conclusion    These results demonstrate the importance of HP0318 gene for adaptive colonization of H. pylori in Mongolian gerbils.

相似文献/References:

[1]乔爱国,何思春,杨丽华,等.根除幽门螺杆菌治疗慢性特发性血小板减少性紫癜的临床探讨[J].陆军军医大学学报(原第三军医大学学报),2007,29(14):1450.
[2]李滨,陈立,杨武晨,等.幽门螺杆菌尿素酶B亚单位优势Th表位的系统筛选与鉴定[J].陆军军医大学学报(原第三军医大学学报),2013,35(03):212.
 Li Bin,Chen Li,Yang Wuchen,et al.Systemic identification of immunodominant Th epitopes from urease B subunit of Helicobacter pylori[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(11):212.
[3]赵晶京,房殿春.幽门螺杆菌感染与Barrett食管关系的研究[J].陆军军医大学学报(原第三军医大学学报),2008,30(04):355.
 ZHAO Jing-jing,FANG Dian-chun.Relationship between Barrett’s oesophagus and Helicobacter pylori infection[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(11):355.
[4]葛迪,郭刚,毛旭虎,等.幽门螺杆菌UreB414-OMP18-LTB融合蛋白的构建及免疫原性初步研究[J].陆军军医大学学报(原第三军医大学学报),2008,30(12):1107.
 GE Di,GUO Gang,MAO Xu-hu,et al.Construction and immunogenicity of a fusion vaccine candidate of UreB-OMP18-LTB from H. pylori[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(11):1107.
[5]兰春慧,房殿春.幽门螺杆菌对胃癌细胞线粒体细胞色素氧化酶亚基Ⅰ表达的影响[J].陆军军医大学学报(原第三军医大学学报),2006,28(03):198.
[6]张卫军,郭刚,刘开云,等.幽门螺杆菌双价亚单位分子内佐剂疫苗的构建表达与纯化[J].陆军军医大学学报(原第三军医大学学报),2008,30(17):1587.
 ZHANG Wei-jun,GUO Gang,LIU Kai-yun,et al.Expression and purification of fusion protein of Helicobacter pylori ahpC-ureB414 and E.coli heat-labile enterotoxin B subunit[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(11):1587.
[7]张卫军,邹全明,毛旭虎,等.幽门螺杆菌疫苗候选抗原lpp20基因的克隆表达及其纯化研究[J].陆军军医大学学报(原第三军医大学学报),2006,28(14):1499.
[8]肖兰青,熊小虎.半枝莲中的黄酮抗幽门螺杆菌作用研究[J].陆军军医大学学报(原第三军医大学学报),2011,33(15):1643.
[9]柏杨,邹全明,曾韦锟.pT-ureB及pT-hpaA核酸疫苗诱导小鼠免疫应答的实验研究[J].陆军军医大学学报(原第三军医大学学报),2005,27(23):2321.
[10]周建嫦,张建中,徐采朴,等.幽门螺杆菌cagA基因全长克隆及序列分析[J].陆军军医大学学报(原第三军医大学学报),2005,27(01):36.

更新日期/Last Update: 2008-10-23