[1]杨宪,杨水平,张雪,等.紫杉醇提取工艺的优化试验研究[J].陆军军医大学学报(原第三军医大学学报),2007,29(19):1886-1888.
 YANG Xian,YANG Shui-ping,ZHANG Xue,et al.Optimization of taxol extraction from Taxus baccata L.[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(19):1886-1888.
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紫杉醇提取工艺的优化试验研究(/HTML )
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陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
29卷
期数:
2007年第19期
页码:
1886-1888
栏目:
论著
出版日期:
2007-10-15

文章信息/Info

Title:
Optimization of taxol extraction from Taxus baccata L.
作者:
杨宪杨水平张雪谭劼
西南大学资源环境学院; 重庆赛诺生物药业股份有限公司
Author(s):
YANG XianYANG Shui-pingZHANG XueTAN Jie
College of Resource and Environment, Southwest University, Chongqing 400716; Chongqing Sino Biology Pharmaceutical Co. LTD., Chongqing 410041, China
关键词:
红豆杉紫杉醇HPLC正交试验
Keywords:
Taxus baccatal L.taxolHPLCorthogonal test
分类号:
R282.71; R284.2
文献标志码:
A
摘要:
目的  优化并建立红豆杉树皮中紫杉醇的提取工艺。 方法  以使用RP-HPLC测定的紫杉醇含量为指标,采用正交试验法对提取过程中乙醇浓度(A)、乙醇用量(B)、提取次数(C)、提取时间(D)4因素进行优选试验。 结果  从红豆杉中提取紫杉醇的最主要影响因素是乙醇浓度及乙醇用量,高浓度、适当多的用量有利于提取;提取时间和次数也极为重要,单因素效果分别以每次提取2 h和共提取2次为最佳。 结论  T9(A3B3C2D1)、T8(A3B2C1D3)为优化选出的紫杉醇最佳提取工艺条件。
Abstract:
Objective    To optimize and establish the extractive technology of taxol from Taxus baccatal L..     Methods    HPLC was applied to determine the content of taxol. The orthogonal test was adopted to examine the effects of the 4 factors consisting of ethanol concentration, ethanol volume, extractive times and extractive time.     Results    Ethanol concentration and ethanol volume were essential factors influencing the extraction of taxol. High concentration and amount of ethanol facilitated the extraction. Extractive times and extractive time were also important. By single-factorial effect, 2 h and 2 times in each extraction of taxol were the best.     Conclusion    The optimum extractive condition is T9 (A3B3C2D1) and T8 (A3B2C1D3).

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更新日期/Last Update: 2008-07-15