[1]王璐,徐凌,梁星,等.成骨生长肽对大鼠骨髓基质细胞增殖分化的影响[J].第三军医大学学报,2007,29(18):1783-1786.
 WANG Lu,XU Ling,LIANG Xing,et al.Osteoblastic growth peptide regulates proliferation and differentiation of rat bone marrow stromal cells[J].J Third Mil Med Univ,2007,29(18):1783-1786.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
29卷
期数:
2007年第18期
页码:
1783-1786
栏目:
论著
出版日期:
2007-09-30

文章信息/Info

Title:
Osteoblastic growth peptide regulates proliferation and differentiation of rat bone marrow stromal cells
作者:
王璐徐凌梁星任乃华付钢李苏伶江晓峰
重庆医科大学附属口腔医院修复科;四川大学华西口腔医院修复科
Author(s):
WANG Lu XU Ling LIANG Xing REN Nai-hua FU Gang  LI Su-ling JIANG Xiao-feng
Department of Prosthodontics, Affiliated Hospital of Stomatology, Chongqing University of Medical Sciences, Chongqing 400015;Department of Prosthodontics, West China School of Stomatology, Sichuan University, Chengdu 600041, China
关键词:
成骨生长肽骨髓基质细胞细胞增殖碱性磷酸酶核心结合因子1
Keywords:
osteogenic growth peptidebone marrow stromal cellsproliferationalkaline phosphataseCbfa1
分类号:
R322.71;R329.28;R341
文献标志码:
A
摘要:
目的  研究成骨生长肽(osteogenic growth peptide, OGP)在体外对骨髓基质细胞增殖和成骨向分化的影响。 方法  在体外培养的大鼠骨髓基质细胞中加入不同浓度的OGP(10-11~10-7 mol/L),用MTT法检测细胞增殖,酶联免疫法检测细胞内碱性磷酸酶(ALP)的表达,RT-PCR法检测细胞内核心结合因子1(Cbfa1) mRNA的表达。 结果  OGP对骨髓基质细胞增殖有促进作用(P<0.05),最大效应浓度为10-9 mol/L;能增加细胞内ALP活性(P<0.05),最大效应浓度为10-8 mol/L;可诱导Cbfa1 mRNA的表达(P<0.05)。 结论 OGP可促进骨髓基质细胞的增殖,并通过诱导其内Cbfa1 mRNA的表达促进其成骨向的分化。
Abstract:
Objective    To elucidate the effect of osteogenic growth peptide(OGP) on bone marrow stromal cells proliferation and differentiation in vitro.     Methods    Bone marrow stromal cells were cultured individually in medium contained various concentrations of OGP; and cell proliferation quantity, alkaline phosphatase activity and level of Cbfa1 mRNA were examined by means of MTT,  enzyme-linked immunoreaction and reverse transfer polymerase chain reaction respectively.     Results   In vitro, the addition of OGP in cultures resulted in an increase of cell proliferation(P<0.05), ALP activity(P<0.05) and level of mRNA for Cbfa1(P<0.05). All concentrations (10-11- 10-7 mol/L) of OGP were effective but the maximum response was obtained with 10-8 mol/L OGP.     Conclusion    Our results demonstrate that OGP stimulates proliferation and sequential differentiation of bone marrow stromal cells in vitro.

参考文献/References:

王璐,徐凌,梁星,等.  成骨生长肽对大鼠骨髓基质细胞增殖分化的影响[J].第三军医大学学报,2007,29(18):1783-1786.

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更新日期/Last Update: 2008-07-16